Influence of Al³⁺ on the titer of spiramycin and effective components in fermentor

ABSTRACT

Spiramycin is a multicomponent antibiotic, and different components have different antibacterial activities. In Streptomyces spiramyceticus 16-10-2, spiramycin II and spiramycin III (SPMII and SPMIII) are the main components, while spiramycin I (SPMI) needs to be controlled below 12%. Based on this, the influences of Al³⁺ on total spiramycin titer and components were investigated in this work. Those experiments were mainly performed in 15 L fermentor and Al³⁺ made a great improvement in spiramycin titer. The optimal adding concentration and adding time of Al³⁺ were 0.32 g/L at 12 hr. Under this condition, spiramycin titer was increased by 19.51% compared with the control. Moreover, the percentage of SPMII and SPMIII was increased by 7.14%. At the same time, the time of mycelia autolysis was lengthened. In addition, the specific activities of acetyl-CoA synthetase, acetate kinase, acetylphosphotransferase, and acylating enzyme were much higher than those of control. The content of acetic acid and succinic acid was beyond 3 and 4.5 times than that of control, respectively.

KEYWORDS:

• Al³⁺
• Streptomyces spiramyceticus 16-10-2
• Spiramycin

Acknowledgments

This work was financially supported by Topfond Pharmaceutical Co., Ltd, Henan, China.