Effects of zinc on expression of apoptosis-related genes in freezing thawing damage of adipose tissue derived mesenchymal stromal cells

Abstract

The present study was performed to investigate the effects of zinc supplementation on freezing thawing damage in adipose tissue-derived mesenchymal stromal cells (MSC) of mice through studying cellular viability and gene expression profile of apoptosis. Slow freezing method was conducted and the samples were treated with zinc doses 0, 2.5, 5, 10, 25, 50 and 100 µM. Viability was increased in groups of 2.5, 10 and 25 µM zinc in comparison to the control group. Gene expression study showed that in the group of 2.5 µM zinc, Fas, Bax and Caspase3 had down regulation. Up regulation of Bcl2 was observed in the groups of 10 and 25 µM zinc. P53 did not have a protecting regulation in the groups of study. The present study showed that doses 2.5–25 µM of zinc had a rather safe toxicity, increased cellular viability, and ameliorated expression of apoptosis-related genes in both intrinsic and extrinsic pathways.

Keywords:

• Adipose tissue
• apoptosis
• mesenchymal stromal cells
• oxidative stress
• thawing damage
• zinc

Acknowledgments

The present study was extracted from MSc thesis of F Nesari. We thank Hepatitis and Razi Herbal Medicines research centers of Lorestan University of Medical Sciences.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

Data are available from the corresponding author.

Additional information

Funding

The present study was funded by Lorestan University of Medical Sciences.