Antioxidative Activities and Angiotensin I-Converting Enzyme Inhibition of Extracts Prepared from Chum Salmon (Oncorhynchus Keta) Cartilage and Skin

Figures & data

Table 1 The contents of collagen and protein in extracts from chum salmon cartilage and skin.

Sample	Collagen (mg/ml)	Protein (mg/ml)
Extract from cartilage and skin	2.06 ± 0.12	24.1 ± 0.98
Extract from cartilage	0.90 ± 0.09	16.4 ± 0.87
Significant difference (p < 0.05).

Table 2 Antioxidant abilities of extracts from chum salmon cartilage and skin.

Absorbance at 500 nm
	Sample
Time (min)	A	B	C	D	E	F	G	H	I	J	K	L
0	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000	0.000
50	0.099 ± 0.005	0.064 ± 0.003	0.072 ± 0.003	0.111 ± 0.012	0.108 ± 0.007	0.076 ± 0.005	0.068 ± 0.002	0.035 ± 0.008	0.022 ± 0.001	0.016 ± 0.001	0.006	0.379 ± 0.008
100	0.333 ± 0.021	0.262 ± 0.015	0.141 ± 0.010	0.203 ± 0.013	0.329 ± 0.024	0.278 ± 0.011	0.170 ± 0.010	0.081 ± 0.009	0.135 ± 0.002	0.032 ± 0.002	0.025 ± 0.002	0.715 ± 0.025
200	1.462 ± 0.040	1.178 ± 0.031	0.372 ± 0.021	0.343 ± 0.017	1.258 ± 0.038	1.231 ± 0.029	0.590 ± 0.020	0.175 ± 0.009	0.469 ± 0.029	0.090 ± 0.008	0.028 ± 0.002	1.406 ± 0.041
(A) × 1/1000 cartilage and skin extract; (B) × 1/100 cartilage and skin extract; (C) × 1/10 cartilage and skin extract; (D) × 1 cartilage and skin extract; (E) × 1/1000 cartilage extract; (F) × 1/100 cartilage extract; (G) × 1/10 cartilage extract; (H) × 1 cartilage extract; (I) 1 mM ascorbic acid; (J) 5 mM ascorbic acid; (K) 1 mM α-tocopherol; (L) control. Significant differences between the results were determined by ANOVA methodology followed by Fisher's PLSD test. Differences were considered significant for p < 0.05.

Table 3 Superoxide anion radical, hydroxyl radical, and DPPH radical scavenging abilities of extracts from chum salmon cartilage and skin.

	Scavenging activity (%)
Sample	Superoxide anion radical	Hydroxyl radical	DPPH radical
A	0.0	10.1 ± 0.08	0.0
B	0.0	15.4 ± 0.12	0.0
C	0.0	15.4 ± 0.11	2.5 ± 0.02
D	13.2 ± 0.10	67.7 ± 5.02	2.5 ± 0.03
E	0.0	20.0 ± 0.24	0.0
F	0.0	24.1 ± 0.45	0.3
G	0.0	32.4 ± 2.78	5.3 ± 0.09
H	87.9 ± 4.98	89.6 ± 6.04	26.8 ± 1.44
I	13.3 ± 0.14	13.2 ± 0.21	3.1 ± 0.04∗
J	90.0 ± 5.43	16.1 ± 0.64	34.1 ± 2.49∗∗
K	52.5 ± 4.16	67.3 ± 4.33	87.5 ± 6.77
See sample nomenclature in Table 2. ∗0.1 mM ascorbic acid; ∗∗1.0 mM ascorbic acid. Significant differences between the results were determined by ANOVA methodology followed by Fisher's PLSD test. Differences were considered significant for p < 0.05.

Table 4 ACE inhibitory abilities of extracts from chum salmom cartilage and skin.

Sample species	(mg protein) IC50
Extract of cartilage and skin	62.6 ± 1.03
Extract of cartilage	8.87 ± 0.52
Significant difference (p < 0.05).