Isolation and Identification of Antioxidative Compound from Fruit of Mengkudu (Morinda citrifolia L.)

Figures & data

Figure 1 Eluates following Sephadex LH-20 column chromatography of fruit fraction of M. citrifolia.

Table 1 Total phenolic content of fruit extract ^a of Morinda citrifolia.

Pooled fraction	%	Total phenolic content (μg/g) as (+)- catechin equivalents ^b
I	18.40	22.1 ± 10.3 ^a
II	14.50	17.4 ± 1.9 ^ab
III	15.16	18.2 ± 3.9 ^ab
IV	4.29	5.1 ± 0.4 ^b
V	23.80	28.5 ± 3.2 ^a
VI	23.90	28.7 ± 10.5 ^a
^aSeparated on Sephadex LH-20 Column; ^bValues are means ± standard deviation of duplicate analyses. Mean with same letter (A B C) are not significantly different (p < 0.05).

Figure 2 Antioxidative activity of Sephadex LH 20 column chromatographic fractions obtained from fruit extracts of M. citrifolia as measured by FTC method. Absorbance values represent triplicates of different samples analysed. Values with same letter (abc) are not significantly different (p < 0.05), between samples.

Figure 3 Antioxidative activity of Sephadex LH 20 column chromatographic fractions obtained from fruit extracts of M. citrifolia as measured by TBA method. Absorbance values represent triplicates of different samples analysed. Values with same letter (abc) are not significantly different (p < 0.05), between samples.

Figure 4 Chromatogram of fraction I, II, III, IV, V, VI obtained from fruit extracts of M. citrifola monitored at 370 nm. The chromatographic conditions are described in text.

Figure 5 Flavonoid content of Sephadex LH 20 column chromatographic fractions obtained from fruit extracts of M. citrifolia. Values with same letter (ABC) are not significantly different at (p < 0.05).