A Comparative Analysis of Colour Measurements of the Seed Coat and Endosperm of Wheat Kernels Performed by Various Techniques

Figures & data

Figure 1 Cross-section through a wheat kernel (author: Zapotoczny).

Figure 2 Kernel arrangement–scanner (author: Zapotoczny).

Figure 3 Image preparation for measurement (author: Zapotoczny).

Figure 4 Component R for particular varieties.

Figure 5 Component G for particular varieties.

Figure 6 Component B for particular varieties.

Table 1 Correlation for RGB components (linear correlation coefficients between studied parameters – 17 wheat varieties)

Parameter	Rsc	Gsc	Bsc	Ren	Gen	Ben	Rsp	Gsp	Bsp
Rsc	*
Gsc	0.95	*
Bsc	0.76	0.91	*
Ren	0.82	0.76	0.55	*
Gen	0.80	0.74	0.52	1.00	*
Ben	0.80	0.73	0.51	0.99	1.00	*
Rsp	0.89	0.81	0.61	0.77	0.76	0.77	*
Gsp	0.90	0.90	0.79	0.76	0.74	0.75	0.94	*
Bsp	0.72	0.82	0.86	0.55	0.52	0.52	0.69	0.89	*
Critical coefficient of linear correlation r = 0.48 (p < 0.05).
*Significant.

Figure 7 Components X, Y for particular varieties.

Figure 8 Components Z for particular varieties.

Table 2 Correlation for XYZ components (linear correlation coefficients between studied parameters – 17 wheat varieties)

Parameter	Xsc	Ysc	Zsc	Xen	Yen	Zen	Xsp	Ysp	Zsp
Xsc	*
Ysc	1.00	*
Zsc	0.91	0.93	*
Xen	0.78	0.77	0.62	*
Yen	0.78	0.77	0.61	1.00	*
Zen	0.78	0.76	0.60	1.00	1.00	*
Xsp	0.90	0.88	0.77	0.81	0.80	0.81	*
Ysp	0.91	0.90	0.80	0.80	0.80	0.81	1.00	*
Zsp	0.83	0.84	0.87	0.65	0.64	0.64	0.87	0.91	*
Critical coefficient of linear correlation r = 0.48 (p < 0.05).
* Significant.

Figure 9 Component L* for particular varieties.

Table 3 Table of correlation for L*a*b* components, hue and saturation of color components (linear correlation coefficients between studied parameters – 17 wheat varieties)

Parameter	L*sc	a*sc	b*sc	h^0 sc	S*sc	L*en	a*en	b*en	h^0 en	S*en	L*sp	a*sp	b*sp	h^0 sp	S*sp
L* sc	*
a* sc		*
b* sc	0.49	0.75	*
h^0 sc				*
S* sc	0.49	0.75	1.00		*
L* en	0.76		0.65		0.65	*
a* en							*
b* en	−0.56	−0.59	−0.60		−0.60	−0.79		*
h^0 en		−0.50	−0.64		−0.64	−0.71	0.66	0.88	*
S* en	−0.62	−0.57	−0.50		−0.51	−0.74		0.95	0.69	*
L* sp	0.90		0.53		0.53	0.75		−0.64		−0.69	*
a* sp		0.84	0.72		0.72			−0.64	−0.59	−0.59	0.57	*
b* sp	0.63	0.74	0.80		0.80	0.72		−0.71	−0.64	−0.66	0.77	0.92	*
h^0 sp		−0.74										−0.84	−0.56	*
S* sp	0.59	0.77	0.79		0.79	0.68		−0.70	−0.64	−0.66	0.74	0.95	1.00	−0.62	*
Critical coefficient of linear correlation r = 0.48 (p < 0.05).
*Significant.

Figure 10 Component a* for particular varieties.

Figure 11 Component b* for particular varieties.

Figure 12 Color hue for particular varieties.

Figure 13 Color saturation for particular varieties.

Table 4 Results of wheat grain color measurements—hue and saturation

Variety	Color parameters
	Seed coat - image analysis				Endosperm - image analysis				Seed coat - spectrophotometer
	h^0 sc [-]	SEM	S*sc [-]	SEM	h^0 en [-]	SEM	S*en [-]	SEM	h^0 sp[-]	SEM	S*sp[-]	SEM
BANTI	88.78	0.04	24.05	0.06	134.84	0.47	23.26	0.09	69.14	0.15	18.74	0.14
JASNA	88.41	0.04	24.62	0.10	138.74	0.72	24.52	0.11	66.91	0.13	18.90	0.23
KOBRA	88.50	0.06	24.50	0.09	140.47	0.97	24.11	0.13	68.30	0.18	18.63	0.27
KOKSA	87.42	0.06	21.87	0.06	142.98	0.68	26.44	0.17	69.24	0.28	14.73	0.13
KORWETA	87.10	0.11	22.19	0.08	147.81	1.05	24.00	0.18	69.95	0.21	16.00	0.16
MEWA	88.72	0.04	25.70	0.07	130.26	0.61	22.84	0.08	67.75	0.14	18.71	0.14
NAWRA	88.70	0.04	23.66	0.07	141.04	0.94	25.25	0.20	68.58	0.24	16.65	0.16
OPATKA	88.78	0.05	23.19	0.06	155.10	0.75	26.48	0.23	67.60	0.18	16.59	0.12
PEGASSOS	88.41	0.05	25.06	0.08	139.90	0.61	23.66	0.10	68.30	0.09	18.93	0.15
SAKWA	88.44	0.05	24.23	0.08	138.79	0.68	23.60	0.10	69.61	0.23	16.70	0.15
SUKCES	88.00	0.07	24.92	0.08	142.62	1.13	24.08	0.18	68.36	0.16	17.54	0.17
SYMFONIA	87.65	0.09	25.86	0.08	131.69	0.94	23.51	0.16	66.87	0.15	19.67	0.27
TONACJA	88.10	0.05	25.53	0.09	138.41	0.85	23.30	0.13	68.09	0.16	18.16	0.17
TORKA	88.71	0.04	25.03	0.09	134.85	0.63	24.98	0.16	69.02	0.20	16.72	0.13
VINJET	88.40	0.05	25.60	0.07	140.01	0.67	25.69	0.14	69.78	0.10	18.33	0.22
ZEBRA	88.86	0.04	24.56	0.05	147.59	0.86	25.70	0.18	69.87	0.16	16.69	0.10
ZYTA	87.64	0.06	23.55	0.05	148.81	0.80	25.63	0.16	69.82	0.20	15.70	0.13