Selected Properties of Araucaria Angustifolia and Araucaria Araucana Seed Protein

Figures & data

Table 1 Soluble protein content (g soluble protein/100 g total protein) of gametophyte and raw seed flour of A. angustifolia and A. araucana seeds, extracted with different media. Values not followed by the same letter are different at the 5% level

	Gametophyte		Raw seed flour
Media	A. angustifolia	A. araucana	A. angustifolia	A. araucana
Distilled water	67.6	70.4	18.3a	34.5b
Saline solution	7.7	8.1	2.6	3.2
Ethanol	0.8	0.7	0.9	0.8
Borate buffer	1.6	1.3	7.0a	2.7b

Table 2 Soluble protein content (g soluble protein/100 g total protein) of gametophyte and raw seed flour of A. angustifolia and A. araucana seeds, extracted with different media. Values not followed by the same letter are different at the 5% level. DW: distilled water; B: buffer, pH 8.0; BSU: buffer, pH 8.0, containing SDS and urea

	Gametophyte		Raw seed flour
Media	A. angustifolia	A. araucana	A. angustifolia	A. araucana
DW	59.5a	74.3b	17.4a	25.5b
B	49.7a	64.3b	28.7a	36.8b
BSU	102.8a	87.2b	83.9	88.9

Figure 1 Electrophoretic patterns of protein components of embryo, gametophyte and raw flour of araucaria seeds. (a) Raw flour of A. araucana; (b) raw flour of A. angustifolia; (c) gametophyte of A. araucana; (d) gametophyte of A. angustifolia; (e) embryo of A. araucana; (f) embryo of A. angustifolia.

Table 3 Amino acid composition (mg/g protein) of A. angustifolia and A. araucana raw flour proteins. Values not followed by the same letter are different at the 5% level. The essential amino acid composition of casein powder and soy protein isolate, and the suggested requirements of essential amino acids (more than 1 year old) of the FAO/WHO and Institute of Medicine of the National Academy of Sciences (USA) were also included

Amino acid	A. angustifolia seed flour	A. araucana seed flour	Soy protein isolate[20]	Casein[21]	Inst. Med. Nat. Acad. Sci.[22]	FAO/WHO[22]
Ala	48a	61b
Arg	108	111
Asp + Asn	104	96
Cys	20	23
Glu + Gln	185	180
Gly	51	47
His	27	26			18	19
Ile	37	36	44	54	25	28
Leu	72a	68b	68	95	55	66
Lys	64	58	52	81	51	58
Met	24a	27b
Phe	61a	56b
Pro	55	58
Ser	53	51
Thr	38	38	40	47	27	34
Trp	8	6		16	7	11
Tyr	45a	67b
Val	66	62	43	75	32	35
Met + Cys	44	50	10	32	25	25
Phe + Tyr	106	123	88	111	47	63

Table 4 Amino acid composition (moles%) of A. angustifolia and A. araucana seed protein

% Amino acid	A. angustifolia seed flour	A. araucana seed flour
Ala	6.6	8.4
Arg	7.5	7.7
Asx	9.4	8.8
Cys	1.9	2.2
Glx	15.5	15.0
Gly	8.5	7.7
His	2.3	2.2
Ile	3.3	3.3
Leu	6.6	6.2
Lys	5.2	4.8
Met	1.9	2.2
Phe	4.7	4.0
Pro	6.1	6.2
Ser	6.1	5.9
Thr	3.8	4.0
Trp	0.5	0.4
Tyr	3.3	4.4
Val	7.0	6.6

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