Figures & data
Figure 1 SDS-PAGE pattern of PH and PHH prepared with pepsin. (M—standard marker proteins). The positions and molecular masses of standards are indicated on the left.
Figure 2 Antioxidative activity measured in a linoleic acid/FTC model system in the presence of PHH. Higher UV absorbance at 500 nm represents higher lipid peroxidation: (▴) control, (○) 5 mg/ml porcine hemoglobin, (
) 5 mg/ml PHH, (□) 10 mg/ml PHH, (▵) 20 mg/ml PHH, (▿) 40 mg/ml PHH, (⋄) 0.05 mg/ml α-tocopherol, (×) 0.1 mg/ml α-tocopherol, (♦) 0.05 mg/ml BHT.
Figure 3 DPPH radical scavenging activity of PHH: (•) PHH, (▾) ascorbic acid, (▪) BHA.
Figure 4 Superoxide radical scavenging activity of PHH: (•) PHH, (▾) ascorbic acid.
Figure 5 Hydroxyl radical scavenging activity of PHH: (•) PHH, (▾) ascorbic acid.
Figure 6 Reducing power of PHH: (•) PHH, (▾) ascorbic acid, (♦) BHT.
Figure 7 Metal chelating activity of PHH: (•) PHH, (
Figure 8 Synergistic antioxidant effects of PHH. Total weight of PHH and TOH used in the linoleic acid/FTC system was maintained at 0.5 mg and different weight combinations were tested for the antioxidant activity. TOH and PHH were used as positive controls (0.2 mg/ml). Values with different letters are significantly different (P < 0.05).
Figure 9 Synergistic antioxidant mechanisms of PHH and TOH in the linoleic acid/FTC system.
