Assessment of Antioxidant Constituents and Anti-Oxidative Properties of Vegetable Soybean

Figures & data

Figure 1 Separation of daidzein, glycitein, and genistein through HPLC.

Table 1 Antioxidant constituents of immature seeds of 16 vegetable-type soybean genotypes

Genotype	Vitamin (mg/100 g)	Daidzein (mg/100 g)	Glycitein (mg/100 g)	Genistein (mg/100 g)	Total ISF (mg/100 g)	Total phenol (mg GAE/g)
AGS432	66.7^e	7.30^c	0.6^a	6.4^e	14.3^c	1.25^bc
AGS433	54.8^d	7.7^c	2.3^c	2.7^a	12.7^b	1.30^c
AGS434	51.2^c	5.6^bc	1.4^bc	2.6^a	9.7^a	1.18^bc
AGS435	56.3^d	5.3^bc	1.1^b	3.0^bc	9.5^a	1.22^bc
AGS436	58.1^d	4.3^ab	2.2^d	3.2^bc	9.7^a	1.39^c
AGS437	56.8^d	3.8^a	5.3^f	7.6^f	16.7^d	1.33^c
AGS438	41.4^b	7.6^c	4.6^e	1.9^a	14.2^c	1.29^c
AGS439	88.7^h	6.6^c	1.2^bc	2.6^ab	10.5^b	1.17^bc
AGS440	38.2^ab	4.8^ab	1.1^ab	2.8^ab	8.6^a	1.22^bc
Dada-cha 2000	34.8^a	17.2^g	8.5^h	7.5^f	33.2^g	1.36^c
Dada-cha ma-me	44.9^b	15.9^f	5.2^ef	5.9^e	27^f	1.08^b
GC98017-7-196-1-2-BNG	50.6^c	5.2^b	0.9^ab	3.7^cd	9.8^a	1.18^b
GC99010-35-1-2-2-BNG	68.1^f	7.8^c	1.6^c	3.0^b	12.3^b	1.30^c
Kegone	52.8^cd	6.8^c	1.7^c	4.4^d	12.9^bc	0.068^a
ASG328 Kohine	39.0^ab	10.8^d	5.4^f	6.2^e	22.4^e	1.01^b
ASG328 Sricanan	77.9^g	14.0^e	8.0^g	6.0^e	27.9^f	1.05^b
Mean	55.0	8.2	3.2	4.3	15.7	1.19
Values given are mean of triplicate samples. Values with different superscripts in the same column are significantly different from each other at P < 0.05.

Table 2 Antioxidant properties of immature seeds of 16 vegetable-type soybean genotypes

Genotype	FRAP (μmoles/g)	FRSA (% reduction)	β-Carotene bleaching inhibition assay (%)
AGS432	5.19^ab	15.92^cd	77.20^e
AGS433	5.63^ab	19.27^a	47.91^b
AGS434	8.70^c	12.35^ab	66.66^d
AGS435	4.29^a	11.66^ab	50.00^b
AGS436	4.08^a	15.56^cd	39.58^a
AGS437	6.00^b	14.92^b	42.10^ab
AGS438	9.56^cd	14.82^b	52.63^bc
AGS439	5.22^ab	12.90^ab	94.11^f
AGS440	7.13^b	18.08^bc	56.14^c
Dada-cha 2000	10.94^d	23.12^e	72.72^de
Dada-cha ma-me	10.02^cd	24.65^e	90.80^f
GC98017-7-196-1-2-BNG	6.34^b	18.73^c	79.41^e
GC99010-35-1-2-2-BNG	5.16^ab	17.84^bc	68.42^de
Kegone	3.95^a	10.03^a	68.42^de
ASG328 Kohine	9.65^cd	23.76^e	74.55^e
ASG328 Sricanan	9.30^cd	24.31^e	76.36^e
Mean	6.95	17.37	66.06
Values given are mean of triplicate samples. Values with different superscripts in the same column are significantly different from each other at P < 0.05.

Table 3 Significant correlations between antioxidants and antioxidative properties of vegetable-type soybean genotypes

Parameter	FRSA	Daidzein	Glycitein	Genistein	Total isoflavones
FRAP	0.683**	0.734**	0.744***		0.736***
FRSA		0.770***	0.669**	0.499*	0.766***
β-Carotene bleaching inhibition		0.526*
Daidzein			0.769***	0.534*	0.932***
Glycitein				0.651**	0.914***
Genistein					0.757***
^aValues superscripted with*, **, and *** are positively significant at p value <0.01, <0.005, and <0.001, respectively.