Degradation Kinetics of Jujuboside a by Rat Intestinal Flora and Identification of the Metabolites by HPLC-Ms/Ms

Figures & data

Table 1  MS/MS detection parameters for the metabolites of JuA

Name of sample	ESI(+/−)	Qualitative ion	Fragment (V)	Collision energy (V)	Dwell (ms)
MJ1	ESI(−)	1043.2 → 749.6	320	50	200
MJ2	ESI(−)	911.5 → 749.2	340	30	200
MJ3	ESI(−)	898.4 → 808.6	260	30	200
MJ4	ESI(−)	765.4 → 667.2	180	10	200
MJ5	ESI(−)	750.4 → 660.4	130	30	200
MJ6	ESI(−)	603.7 → 379.4	115	30	200
MJ7	ESI(−)	471.6 → 403.1	90	10	200

Table 2  Accuracy of JuA in sample solutions (n = 3)

	Accuracy
Analyte conc. (μg·mL^−1)	Mean detected conc. (μg·mL^−1)	Accuracy (%)	RSD (%)
4.8	4.85	101.00 ± 3.61	3.57
8.8	8.53	96.96 ± 2.70	2.79
12.8	12.32	96.27 ± 0.92	0.96

Figure 1  Representative chromatograms for: (a) MS₂-scan chromatograms of blank GAM solution; (b) MRM chromatograms of the standard of JuA; (c) MRM chromatograms of the sample.

Figure 2  Logarithmic degradation curves of JuA in different concentrations incubated with rat’s feces (—) and negative control (- - - -). (n = 3). (a) 5 μg·mL⁻¹; (b) 10 μg·mL⁻¹; (c) 20 μg·mL⁻¹.

Figure 3  Product ions chromatograms of the metabolites: (a) MJ1; (b) MJ2; (c) MJ4; (d) MJ7.