Effect of Epigallocatechin Gallate on the Properties of Gelatin

Figures & data

Figure 1 Appearance and gel strength values of gelatin hydrogels treated with and without EGCG. Values are means (n = 5) ± standard deviation. Values in columns followed by same letter are not significantly different at α = 0.01.

Figure 2 Calorimetric curves of gelatin xerogels treated with and without EGCG. (0) 0 g/l EGCG; (1) 0.5 g/l EGCG; (2) 1.0 g/l EGCG; (3) 2.0 g/l EGCG; (4) 3.0 g/l EGCG; (5) 4.0 g/l EGCG.

Table 1  The d values of diffraction peaks in X-ray diffraction diagrams of gelatin xerogels treated with and without EGCG

	Peak wavenumber (2θ,°)		d value (Å)
Concentration of EGCG in gelatin gel (g/l)	First peak	Second peak	First peak	Second peak
0.0	6.91E	20.1A	12.78A	4.41F
0.5	7.20D	19.89B	12.27B	4.46E
1.0	7.57A	16.89E	11.65E	5.23A
2.0	7.46B	18.44D	11.84D	4.81B
3.0	7.35C	18.99C	12.10C	4.67C
4.0	7.23D	18.95C	12.16C	4.62D

Different letters in the same column indicate very significant differences (p < 0.01) among samples.

Table 2  Fourier transform infrared absorption band assignment of gelatin hydrogels treated with and without EGCG

	Peak wave number (cm^−1)
Concentration of EGCG in gelatin gel (g/l)	Amide A	Amide I	Amide II	Amide III
0	3294.79B	1629.55A	1543.74A	1237.59A
0.5	3293.82C	1628.59C	1540.36C	1235.67B
1.0	3270.20F	1628.59C	1533.13F	1233.74E
2.0	3285.63E	1629.07B	1535.54E	1235.18C
3.0	3304.91A	1628.59C	1536.02D	1234.70D
4.0	3292.38D	1629.07B	1543.26B	1234.70D

Different letters in the same column indicate very significant differences (p < 0.01) among samples.

Figure 3 X-ray diffraction diagrams of gelatin xerogels treated with and without EGCG. (0) 0 g/l EGCG; (1) 0.5 g/l EGCG; (2) 1.0 g/l EGCG; (3) 2.0 g/l EGCG; (4) 3.0 g/l EGCG; (5) 4.0 g/l EGCG.

Figure 4 Fourier transform infrared spectrum of gelatin xerogels treated with and without EGCG. (0) 0 g/l EGCG; (1) 0.5 g/l EGCG; (2) 1.0 g/l EGCG; (3) 2.0 g/l EGCG; (4) 3.0 g/l EGCG; (5) 4.0 g/l EGCG.

Figure 5 Second derivative of amide I band from FTIR spectra of gelatin xerogels treated with and without EGCG. (0) 0 g/l EGCG; (1) 0.5 g/l EGCG; (2) 1.0 g/l EGCG; (3) 2.0 g/l EGCG; (4) 3.0 g/l EGCG; (5) 4.0 g/l EGCG.

Figure 6 SDS-PAGE analysis of gelatin xerogels treated with and without EGCG. Lane C: Channel catfish skin collagen; Lane 0: 0 g/l EGCG; Lane 1: 0.5 g/l EGCG; Lane 2: 1.0 g/l EGCG; Lane 3: 2.0 g/l EGCG; Lane 4: 3.0 g/l EGCG; Lane 5: 4.0 g/l EGCG.

Figure 7 SEM surface images of gelatin xerogel treated with and without EGCG. (a) 0 g/l EGCG; (b) 0.5 g/l EGCG; (c) 1.0 g/l EGCG; (d) 2.0 g/l EGCG; (e) 3.0 g/l EGCG; (f) 4.0 g/l EGCG.