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Original Articles

Multiclass Determination of Phenolic Compounds in Different Varieties of Tomato and Lettuce by Ultra High Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry

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Pages 494-507 | Received 09 Jun 2014, Accepted 14 Oct 2014, Published online: 02 Dec 2015

Figures & data

TABLE 1 UHPLC-MS/MS parameters

FIGURE 1 Slope ratios between matrix-matched and solvent calibration. Compliance interval covering the range between 0.8 and 1.2 for tolerable matrix effect has been plotted. Phenolic compound codes are indicated in .

FIGURE 1 Slope ratios between matrix-matched and solvent calibration. Compliance interval covering the range between 0.8 and 1.2 for tolerable matrix effect has been plotted. Phenolic compound codes are indicated in Table 1.

TABLE 2 Validation parameters of the optimized UHPLC-MS/MS method in lettuce

TABLE 3. Content of phenolic compounds (mg/kg DW) in different varieties of lettuce

FIGURE 2 Concentration of the phenolic compounds (mg/kg DW) in: (a) lettuce (a) and (b) tomato. Three samples for Trocadero and Mini-Romaine Heart, four samples for Iceberg, five samples for Iceberg fresh-cut, and six samples for Romaine and Mini-Romaine were analyzed in lettuce. For tomato, three samples of each variety were analyzed.

FIGURE 2 Concentration of the phenolic compounds (mg/kg DW) in: (a) lettuce (a) and (b) tomato. Three samples for Trocadero and Mini-Romaine Heart, four samples for Iceberg, five samples for Iceberg fresh-cut, and six samples for Romaine and Mini-Romaine were analyzed in lettuce. For tomato, three samples of each variety were analyzed.

FIGURE 3 UHPLC-MS/MS MRM chromatograms for tomato and lettuce containing: (a) Quercetin at 20 mg/kg DW in trocadero lettuce; (b) Luteolin-O-glucoside at 17 mg/kg in mini-romaine lettuce; (c) Quercetin-3-O-rutinoside at 25 mg/kg DW in plum tomato; and (d) Chlorogenic acid at 317 mg/kg DW in Raf tomato.

FIGURE 3 UHPLC-MS/MS MRM chromatograms for tomato and lettuce containing: (a) Quercetin at 20 mg/kg DW in trocadero lettuce; (b) Luteolin-O-glucoside at 17 mg/kg in mini-romaine lettuce; (c) Quercetin-3-O-rutinoside at 25 mg/kg DW in plum tomato; and (d) Chlorogenic acid at 317 mg/kg DW in Raf tomato.

TABLE 4 Content of phenolic compounds (mg/kg DW) in different varieties of tomato

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