Stability of tea polyphenols solution with different pH at different temperatures

Figures & data

Figure 1. Transmittance in the spectra at 640 nm change of TP solutions with pH 3–7 at A: 4°C; B: at 25°C; C: at 100°C. Data are expressed as means ± SD of n = 3 samples. The different letter in the same temperature indicated that the difference between the treatments is significantly through LSD test (p < 0.05).

Figure 2. Absorbance in the spectra at 425 nm change of TP with pH 3–7 at A: 4°C; B: at 25°C; C: at 100°C. Data are expressed as means ± SD of n = 3 samples. The different letter in the same temperature indicated that the difference between the treatments is significantly through LSD test (p < 0.05).

Table 1a. Color change of TP solution with pH 3–7 at 4°C.

Color change	pH	0 h	1 h	3 h	5 h	10 h	24 h
L*	3	92.00 ± 1.0	92.13 ± 0.5	92.10 ± 0.4	92.01 ± 0.4	92.31 ± 0.4	91.93 ± 0.2
		0a	1a	7a	1a	9a	2a
	4	92.00 ± 1.0	91.74 ± 0.4	91.77 ± 0.3	91.80 ± 0.5	92.11 ± 0.7	91.83 ± 0.3
		0a	8a	5a	8a	3a	2a
	5	92.00 ± 1.0	91.56 ± 0.1	91.67 ± 0.4	91.51 ± 0.5	91.90 ± 0.3	91.49 ± 0.1
		0a	7a	5a	1a	3a	2a
	6	92.00 ± 1.0	90.98 ± 0.1	91.21 ± 0.3	91.31 ± 0.4	91.50 ± 0.3	91.23 ± 0.1
		0a	1b	2ab	3ab	8ab	0ab
	7	92.00 ± 1.0	89.18 ± 0.1	88.77 ± 0.7	88.61 ± 0.5	89.02 ± 0.3	88.56 ± 0.5
		0a	5b	0b	4b	2b	1b
a*	3	1.73 ± 0.15	1.89 ± 0.14	1.93 ± 0.10	1.95 ± 0.09	1.93 ± 0.16	1.78 ± 0.27
		a	a	a	a	a	a
	4	1.73 ± 0.15	2.13 ± 0.40	2.04 ± 0.21	2.10 ± 0.27	2.10 ± 0.31	1.95 ± 0.23
		a	a	a	a	a	a
	5	1.73 ± 0.15	2.31 ± 0.09	2.22 ± 0.10	2.18 ± 0.12	2.19 ± 0.09	1.97 ± 0.25
		b	a	ab	ab	ab	b
	6	1.73 ± 0.15	2.82 ± 0.16	2.66 ± 0.14	2.53 ± 0.12	2.55 ± 0.08	2.27 ± 0.29
		c	a	a	ab	ab	b
	7	1.73 ± 0.15	4.12 ± 0.21	4.16 ± 0.06	4.24 ± 0.05	4.41 ± 0.16	4.11 ± 0.18
		c	b	ab	ab	a	b
b*	3	11.17 ± 1.2	12.24 ± 0.2	12.08 ± 0.1	12.09 ± 0.2	11.91 ± 0.4	11.81 ± 0.2
		6b	9a	9ab	9ab	2ab	1ab
	4	11.17 ± 1.2	11.73 ± 0.7	11.82 ± 1.0	11.57 ± 0.2	11.26 ± 0.2	11.39 ± 0.0
		6a	1a	5a	0a	0a	9a
	5	11.17 ± 1.2	11.66 ± 0.7	11.61 ± 0.6	11.82 ± 0.7	11.18 ± 0.7	11.44 ± 0.4
		6a	5a	7a	2a	9a	7a
	6	11.17 ± 1.2	10.78 ± 0.6	10.78 ± 0.6	10.71 ± 0.2	10.64 ± 0.3	11.02 ± 0.1
		6a	5a	5a	7a	2a	0a
	7	11.17 ± 1.2	11.19 ± 0.3	11.19 ± 0.3	11.93 ± 0.0	12.81 ± 0.1	13.14 ± 0.3
		6b	2b	2b	7b	5ab	9a

Table 1b. Color change of TP solution with pH 3–7 at 25°C.

Color change	pH	0 h	1 h	3 h	5 h	10 h	24 h
L*	3	92.00 ± 1.0	92.23 ± 0.5	92.19 ± 0.4	92.11 ± 0.5	92.28 ± 0.3	91.88 ± 0.2
		0a	2a	6a	4a	5a	8a
	4	92.00 ± 1.0	91.91 ± 0.6	91.86 ± 0.5	91.85 ± 0.4	92.12 ± 0.5	91.78 ± 0.3
		0a	8a	0a	8a	5a	0a
	5	92.00 ± 1.0	91.79 ± 0.3	91.66 ± 0.4	91.77 ± 0.3	91.93 ± 0.2	91.58 ± 0.1
		0a	5a	0a	5a	7a	7a
	6	92.00 ± 1.0	91.30 ± 0.4	91.33 ± 0.3	91.14 ± 0.2	91.47 ± 0.3	91.14 ± 0.2
		0a	0a	9a	3a	6a	5a
	7	92.00 ± 1.0	89.16 ± 0.5	88.63 ± 0.6	87.92 ± 0.7	84.41 ± 0.6	79.97 ± 1.2
		0a	8b	0b	0b	6c	6d
a*	3	1.73 ± 0.15	1.68 ± 0.12	1.77 ± 0.07	1.88 ± 0.17	1.79 ± 0.10	1.82 ± 0.23
		a	a	a	a	a	a
	4	1.73 ± 0.15	1.97 ± 0.38	1.96 ± 0.13	1.88 ± 0.13	1.76 ± 0.10	1.71 ± 0.18
		a	a	a	a	a	a
	5	1.73 ± 0.15	2.06 ± 0.05	1.99 ± 0.07	1.95 ± 0.13	1.86 ± 0.16	1.73 ± 0.22
		b	a	a	ab	ab	b
	6	1.73 ± 0.15	2.61 ± 0.14	2.42 ± 0.09	2.35 ± 0.09	2.14 ± 0.08	1.98 ± 0.15
		d	a	ab	b	bc	c
	7	1.73 ± 0.15	4.51 ± 0.22	4.49 ± 0.15	4.55 ± 0.23	6.00 ± 0.48	8.20 ± 0.87
		d	c	c	c	b	a
b*	3	11.17 ± 1.2	12.10 ± 0.3	12.09 ± 0.1	11.99 ± 0.3	12.17 ± 0.2	12.11 ± 0.5
		6a	2a	7a	3a	0a	6a
	4	11.17 ± 1.2	11.40 ± 0.2	11.39 ± 0.1	11.61 ± 0.1	11.74 ± 0.3	11.86 ± 0.5
		6a	4a	9a	5a	9a	0a
	5	11.17 ± 1.2	11.24 ± 0.8	11.33 ± 0.6	11.44 ± 0.7	11.64 ± 0.7	11.92 ± 0.5
		6a	2a	3a	6a	2a	7a
	6	11.17 ± 1.2	10.43 ± 0.2	10.73 ± 0.1	11.26 ± 0.5	11.74 ± 0.5	12.34 ± 0.7
		6ab	7b	7b	5ab	7ab	3a
	7	11.17 ± 1.2	11.82 ± 0.3	13.25 ± 0.3	15.19 ± 0.9	21.68 ± 1.4	28.99 ± 2.4
		6d	0d	6cd	7c	2b	2a

Table 1c. Color change of TP solution with pH 3–7 at 100°C.

Color change	pH	0 h	1 h	3 h	5 h	10 h	24 h
L*	3	92.00 ± 1.	91.23 ± 0.2	90.77 ± 0.2	90.35 ± 0.1	90.43 ± 1.0	88.43 ± 0.1
		00a	0a	5b	1b	2b	9c
	4	92.00 ± 1.	91.22 ± 0.2	90.61 ± 0.1	90.23 ± 0.2	89.96 ± 0.6	86.56 ± 0.1
		00a	5ab	9b	8b	8b	1c
	5	92.00 ± 1.	89.56 ± 0.3	88.43 ± 0.6	87.80 ± 0.5	88.19 ± 1.4	84.47 ± 1.5
		00a	5b	9b	0b	1b	4c
	6	92.00 ± 1.	88.22 ± 0.5	86.71 ± 1.1	85.92 ± 0.7	85.30 ± 2.1	77.48 ± 0.7
		00a	3b	4bc	6c	8c	9d
	7	92.00 ± 1.	85.30 ± 0.2	81.49 ± 0.4	73.22 ± 1.2	59.54 ± 0.8	43.11 ± 6.5
		00a	8b	7b	1c	6d	5e
a*	3	1.73 ± 0.1	1.93 ± 0.19	1.91 ± 0.08	2.18 ± 0.09	2.38 ± 0.32	3.39 ± 0.18
		5c	c	c	bc	b	a
	4	1.73 ± 0.1	1.76 ± 0.11	1.59 ± 0.07	1.78 ± 0.07	2.02 ± 0.35	3.82 ± 0.12
		5bc	bc	c	bc	b	a
	5	1.73 ± 0.1	2.29 ± 0.14	2.46 ± 0.16	2.80 ± 0.19	2.76 ± 0.64	4.66 ± 0.89
		5c	bc	b	b	b	a
	6	1.73 ± 0.1	2.91 ± 0.51	3.54 ± 0.50	4.18 ± 0.32	4.91 ± 1.38	10.18 ± 0.6
		5d	cd	c	bc	b	1a
	7	1.73 ± 0.1	5.34 ± 0.52	10.33 ± 0.3	21.07 ± 1.0	36.40 ± 0.8	37.56 ± 2.2
		5d	d	2c	5b	8a	7a
b*	3	11.17 ± 1.	14.42 ± 0.1	15.95 ± 0.5	17.83 ± 0.4	19.49 ± 2.1	26.28 ± 0.9
		26d	9c	1c	2bc	0b	1a
	4	11.17 ± 1.	14.32 ± 0.4	16.28 ± 0.0	17.38 ± 0.4	18.69 ± 1.2	25.57 ± 0.6
		26e	9d	8c	7bc	4b	2a
	5	11.17 ± 1.	17.77 ± 0.4	19.99 ± 1.0	21.38 ± 1.0	21.20 ± 1.7	26.50 ± 2.7
		26d	0c	1bc	2b	4b	1a
	6	11.17 ± 1.	19.81 ± 1.0	22.35 ± 1.6	24.19 ± 1.2	25.41 ± 3.6	35.59 ± 1.0
		26d	2c	3bc	6b	2b	6a
	7	11.17 ± 1.	23.37 ± 0.4	25.36 ± 0.3	31.19 ± 1.5	42.17 ± 1.6	46.83 ± 2.7
		26e	7d	8d	5c	8b	7a

Values are expressed as means ± standard deviations of triplicate measurements.

Different superscript lowercase letters (a, b, c, d) in same row indicate that the difference between the treatments is significantly through LSD test (p < 0.05).

T = temperatures.

Table 2a. Change in contents of catechins of TP solution with pH 3–7 at 4°C.

Compounds	pH	0 h	1 h	3 h	5 h	10 h	24 h
C	3		3.06 ± 0.58	2.97 ± 0.86	2.57 ± 0.53
		2.97 ± 0.55a	a	a	a	2.75 ± 0.51a	2.24 ± 0.66a
	4		2.72 ± 0.57	3.16 ± 0.12	3.13 ± 0.18
		2.97 ± 0.55a	a	a	a	3.24 ± 0.26a	3.01 ± 0.33a
	5		3.39 ± 0.21	3.15 ± 0.22	3.33 ± 0.18
		2.97 ± 0.55a	a	a	ab	3.29 ± 0.06ab	3.06 ± 0.07b
	6		3.14 ± 0.11	2.96 ± 0.39	3.13 ± 0.11
		2.97 ± 0.55a	a	a	a	3.04 ± 0.21a	3.10 ± 0.15a
	7		3.14 ± 0.47	3.21 ± 0.52	3.32 ± 0.34
		2.97 ± 0.55a	a	a	a	3.15 ± 0.88a	2.99 ± 0.80a
EC	3	10.17 ± 1.04	10.64 ± 0.1	9.93 ± 0.97	10.34 ± 0.7		10.39 ± 0.39
		a	7a	a	7a	10.39 ± 0.51a	a
	4	10.17 ± 1.04	9.12 ± 2.04	10.26 ± 0.4	10.12 ± 0.4
		a	a	4a	6a	9.70 ± 0.65a	9.98 ± 0.92a
	5	10.17 ± 1.04	9.90 ± 1.01	9.72 ± 0.93	9.78 ± 0.73
		a	a	a	a	8.87 ± 1.36a	8.91 ± 1.19a
	6	10.17 ± 1.04	10.71 ± 0.9	10.50 ± 0.9	10.37 ± 0.9		10.39 ± 0.82
		a	6a	3a	2a	10.44 ± 0.75a	a
	7	10.17 ± 1.04	9.74 ± 0.45	9.79 ± 0.46	9.61 ± 0.36
		a	a	a	a	9.82 ± 0.65a	9.06 ± 0.64a
EGC	3	17.00 ± 1.00	18.10 ± 0.9	18.20 ± 0.6	17.10 ± 0.7		16.71 ± 1.32
		a	6a	0a	2a	17.40 ± 0.69a	a
	4	17.00 ± 1.00	17.10 ± 0.4	16.83 ± 1.4	16.92 ± 1.4		17.03 ± 0.79
		a	5a	6a	1a	17.20 ± 1.88a	a
	5	17.00 ± 1.00	17.87 ± 0.5	17.58 ± 0.5	18.47 ± 0.3		18.69 ± 1.07
		b	9ab	9ab	8a	18.89 ± 0.91a	a
	6	17.00 ± 1.00	17.31 ± 1.0	17.25 ± 1.8	17.75 ± 0.9		18.10 ± 0.94
		a	3a	0a	0a	17.70 ± 0.69a	a
	7	17.00 ± 1.00	14.19 ± 0.3	14.28 ± 0.4	13.06 ± 0.4	12.43 ± 0.71c	11.56 ± 0.42
		a	2b	4b	0c	d	d
ECG	3	60.07 ± 4.90	59.71 ± 4.7	59.92 ± 4.1	60.62 ± 4.3		61.61 ± 4.75
		a	5a	0a	6a	60.50 ± 4.05a	a
	4	60.07 ± 4.90	58.96 ± 5.0	59.45 ± 4.3	59.80 ± 4.3		61.29 ± 2.59
		a	2a	4a	7a	61.48 ± 3.78a	a
	5	60.07 ± 4.90	61.42 ± 4.4	60.29 ± 4.7	57.90 ± 1.9		59.31 ± 1.73
		a	5a	8a	7a	57.84 ± 1.69a	a
	6	60.07 ± 4.90	57.63 ± 6.3	56.44 ± 3.6	56.37 ± 3.8		59.52 ± 0.94
		a	6a	1a	6a	59.25 ± 1.34a	a
	7	60.07 ± 4.90	53.52 ± 1.3	54.44 ± 1.9	54.22 ± 1.4		54.85 ± 3.34
		a	2b	5b	0b	53.44 ± 1.86b	b
GCG	3	18.57 ± 1.50	19.37 ± 1.6	18.08 ± 1.2	19.48 ± 1.2		19.65 ± 0.81
		a	4a	3a	3a	18.80 ± 1.33a	a
	4	18.57 ± 1.50	17.85 ± 2.3	20.49 ± 2.1	20.44 ± 1.8		19.42 ± 2.25
		a	7a	9a	9a	20.87 ± 1.40a	a
	5	18.57 ± 1.50	18.98 ± 1.0	18.60 ± 0.9	19.06 ± 1.1		20.21 ± 0.88
		a	3a	3a	4a	19.36 ± 1.44a	a
	6	18.57 ± 1.50	18.62 ± 1.7	18.68 ± 1.9	18.74 ± 1.8		19.82 ± 0.62
		a	8a	2a	9a	19.48 ± 0.71a	a
	7	18.57 ± 1.50	17.25 ± 0.9	17.40 ± 0.7	17.02 ± 0.8	17.65 ± 0.61a	16.27 ± 1.30
		a	3ab	1ab	9ab	b	b
EGCG	3	279.67 ± 10.50a	284.57 ± 7.50a	282.34 ± 9.22a	280.16 ± 7.27a	275.71 ± 6.14a	281.50 ± 2.96a
	4	279.67 ± 10.	281.08 ± 4.22a	280.81 ± 4.09a	281.95 ± 3.	286.08 ± 10.57a	274.66 ± 11.63a
		50a			43a
	6
	5	279.67 ± 10.50a	275.20 ± 2.96ab	268.61 ± 3.99b	273.29 ± 2.65ab	276.69 ± 0.92ab	277.27 ± 1.74ab
	6	279.67 ± 10.50a	270.58 ± 15.87a	276.17 ± 6.56a	277.28 ± 8.96a	275.84 ± 8.32a	275.96 ± 7.99a
	7	279.67 ± 10.	242.69 ± 11	242.45 ± 10	234.30 ± 13	230.32 ± 15.4	210.49 ± 9.8
		50a	.54b	.57b	.54b	2bc	4c
Catechins	3	388.43 ± 16.85a	395.45 ± 12.36a	391.43 ± 10.42a	390.27 ± 14.12a	385.54 ± 9.91a	392.10 ± 5.93a
	4	388.43 ± 16.85a	386.84 ± 2.44a	391.02 ± 5.51a	392.37 ± 3.88a	398.57 ± 13.49a	385.39 ± 13.24a
		388.43 ± 16.	386.77 ± 1.	377.96 ± 8.	381.83 ± 5.	384.93 ± 3.06	387.46 ± 3.3
		85a	45a	90a	60a	a	8a
	5
	6	388.43 ± 16.	377.98 ± 25	382.01 ± 14	383.62 ± 16	385.75 ± 10.1	386.89 ± 8.0
		85a	.51a	.78a	.55a	9a	0a
	7	388.43 ± 16.	340.53 ± 12	341.57 ± 10	331.53 ± 14	326.82 ± 15.9	305.21 ± 9.9
		85a	.84b	.77b	.49b	8bc	9c

Table 2b. Change in contents of catechins of TP solution with pH 3–7 at 25°C.

Compounds	pH	0 h	1 h	3 h	5 h	10 h	24 h
C	3	2.97 ± 0.55	2.82 ± 0.42	2.67 ± 0.62	2.57 ± 0.47	2.99 ± 1.08
		a	a	a	a	a	2.66 ± 0.49a
	4	2.97 ± 0.55	2.61 ± 0.53	3.13 ± 0.09	3.08 ± 0.17	3.12 ± 0.21
		a	a	a	a	a	2.51 ± 0.78a
	5	2.97 ± 0.55	3.09 ± 0.35	2.81 ± 0.48	3.25 ± 0.15	3.16 ± 0.31
		a	a	a	a	a	2.98 ± 0.11a
	6	2.97 ± 0.55	3.22 ± 0.10	2.69 ± 0.99	3.10 ± 0.29	2.84 ± 0.08
		a	a	a	a	a	2.77 ± 0.37a
	7	2.97 ± 0.55	4.23 ± 0.86	3.83 ± 0.62	4.74 ± 0.27	4.53 ± 0.12
		a	a	a	a	ab	5.71 ± 0.51a
EC	3	10.17 ± 1.0	10.61 ± 0.0	10.00 ± 0.9	10.28 ± 0.7	9.70 ± 0.50	10.01 ± 0.75
		4a	9a	6a	0a	a	a
	4	10.17 ± 1.0	8.84 ± 1.93	10.11 ± 0.4	9.52 ± 0.06	9.17 ± 0.67
		4a	a	5a	a	a	9.48 ± 1.34a
	5	10.17 ± 1.0	9.80 ± 0.96	9.00 ± 0.12	9.32 ± 0.64	8.32 ± 0.80
		4a	ab	ab	ab	b	8.42 ± 0.76b
	6	10.17 ± 1.0	10.27 ± 1.0	9.85 ± 0.74	9.24 ± 0.92	9.35 ± 0.31
		4a	1a	a	b	b	8.35 ± 0.75b
	7	10.17 ± 1.0	9.78 ± 0.50	9.22 ± 0.28	8.95 ± 0.28	8.99 ± 0.45
		4a	ab	ab	b	b	8.08 ± 0.84b
EGC	3	17.00 ± 1.0	17.45 ± 0.7	17.38 ± 0.9	17.09 ± 0.6	17.71 ± 2.0	17.63 ± 1.95
		0a	1a	9a	2a	7a	a
	4	17.00 ± 1.0	16.87 ± 1.2	16.92 ± 1.5	16.83 ± 1.4	16.90 ± 1.6	17.05 ± 1.16
		0a	3a	5a	0a	8a	a
	5	17.00 ± 1.0	17.11 ± 0.4	16.73 ± 1.2	18.27 ± 0.2	18.38 ± 1.2	18.20 ± 1.67
		0a	6a	6a	2a	6a	a
	6	17.00 ± 1.0	16.97 ± 1.0	16.95 ± 1.2	17.43 ± 1.1	16.56 ± 0.0	15.84 ± 2.00
		0a	5a	8a	7a	3a	a
	7	17.00 ± 1.0	14.12 ± 0.8	10.25 ± 0.7	15.63 ± 0.5	13.80 ± 1.5
		0a	4b	9c	9ab	1b	9.22 ± 0.87c
ECG	3	60.07 ± 4.9	61.16 ± 4.6	61.07 ± 4.3	60.22 ± 4.3	61.67 ± 4.7	61.00 ± 4.24
		0a	2a	7a	0a	0a	a
	4	60.07 ± 4.9	59.38 ± 4.5	59.36 ± 4.4	59.98 ± 4.2	60.37 ± 3.4	59.34 ± 3.38
		0a	8a	4a	3a	5a	a
	5	60.07 ± 4.9	61.47 ± 5.0	60.00 ± 3.0	58.48 ± 1.3	58.23 ± 1.1	61.00 ± 2.56
		0a	3a	9a	4a	2a	a
	6	60.07 ± 4.9	56.77 ± 3.6	56.20 ± 3.6	56.50 ± 3.0	59.93 ± 3.5	60.57 ± 1.27
		0a	0a	1a	9a	2a	a
	7	60.07 ± 4.9	58.56 ± 4.1	53.20 ± 1.2	52.66 ± 1.8	48.34 ± 2.4	47.49 ± 2.88
		0a	4ab	9b	7b	1b	c
GCG	3	18.57 ± 1.5	19.32 ± 1.6	17.95 ± 1.1	19.67 ± 1.0	19.19 ± 0.4	19.89 ± 0.69
		0a	5a	6a	4a	4a	a
	4	18.57 ± 1.5	17.41 ± 2.0	20.43 ± 2.2	20.69 ± 1.6	20.18 ± 1.6	20.07 ± 1.57
		0ab	7b	4ab	8a	4ab	ab
	5	18.57 ± 1.5	19.07 ± 1.0	18.73 ± 0.4	19.37 ± 1.0	19.45 ± 1.3	20.49 ± 0.71
		0b	7ab	8ab	0ab	2ab	a
	6	18.57 ± 1.5	20.09 ± 2.2	18.58 ± 1.9	19.55 ± 2.3	20.15 ± 1.9	19.51 ± 0.83
		0a	4a	6a	8a	6a	a
	7	18.57 ± 1.5	17.29 ± 0.6	16.03 ± 0.1	15.63 ± 1.2	14.32 ± 1.2	9.00 ± 0.63c
		0a	1ab	1b	6b	3b
EGCG	3	279.67 ± 10.50a	283.32 ± 8.56a	283.05 ± 7.51a	280.60 ± 6.38a	275.65 ± 4.05a	276.89 ± 5.79a
	4	279.67 ± 10	278.83 ± 1.	280.24 ± 2.	282.41 ± 2.	283.38 ± 4.	280.17 ± 3.6
		.50a	47a	65a	69a	31a	7a
	5	279.67 ± 10	282.18 ± 3.	273.47 ± 6.	282.18 ± 7.	282.02 ± 1.	279.58 ± 2.7
		.50a	48a	78a	18a	85a	1a
	6	279.67 ± 10.50a	267.28 ± 6.83a	268.12 ± 7.33a	266.11 ± 9.31a	266.88 ± 7.23a	266.51 ± 10.87a
	7	279.67 ± 10	240.09 ± 10	196.91 ± 13	166.75 ± 15	146.60 ± 10	47.34 ± 2.25
		.50a	.48b	.50c	.85d	.32e	f

Table 2c. Change in contents of catechins of TP solution with pH 3–7 at 100°C.

Compounds	pH	0 h	1 h	3 h	5 h	10 h	24 h
C	3	2.97 ± 0.55	2.77 ± 0.12			6.23 ± 0.30	7.14 ± 0.60
		c	c	3.01 ± 0.24c	5.85 ± 0.60b	b	a
	4	2.97 ± 0.55	2.84 ± 0.14			5.98 ± 1.48	6.71 ± 0.23
		c	c	4.29 ± 0.43b	5.31 ± 0.50b	ab	a
	5	2.97 ± 0.55	3.31 ± 0.06			6.61 ± 1.12	7.53 ± 0.52
		d	cd	4.33 ± 0.67c	5.87 ± 0.37b	ab	a
	6	2.97 ± 0.55	5.31 ± 0.53			8.34 ± 1.05	9.44 ± 2.00
		c	b	7.76 ± 0.32a	8.71 ± 0.94a	a	a
	7	2.97 ± 0.55	10.54 ± 0.5	9.49 ± 2.18a		5.48 ± 0.96	3.43 ± 0.97
		b	1a	b	12.07 ± 5.32a	b	b
EC	3	10.17 ± 1.0	11.98 ± 0.8	12.20 ± 0.29	11.46 ± 0.32a	10.18 ± 1.1	9.32 ± 1.52
		4b	2a	a	b	8b	b
	4	10.17 ± 1.0	9.36 ± 0.12			9.85 ± 0.72	9.24 ± 0.55
		4a	a	9.45 ± 0.08a	9.90 ± 0.29a	a	a
	5	10.17 ± 1.0	8.67 ± 0.27			8.23 ± 0.48	5.70 ± 1.05
		4a	b	8.54 ± 0.26b	8.63 ± 0.32b	b	c
	6	10.17 ± 1.0	8.78 ± 0.82			4.51 ± 0.84	5.20 ± 2.45
		4a	a	6.06 ± 1.36b	5.55 ± 1.05b	b	b
	7	10.17 ± 1.0	5.33 ± 0.52	4.45 ± 0.50b		4.12 ± 0.17	1.81 ± 0.18
		4a	b	c	4.28 ± 0.67bc	c	d
EGC	3	17.00 ± 1.0	17.63 ± 0.7	16.33 ± 6.60		14.93 ± 2.5	14.46 ± 2.5
		0a	5a	a	14.69 ± 1.06a	2a	0a
	4	17.00 ± 1.0	16.43 ± 1.0	14.06 ± 0.58	14.46 ± 0.30	11.85 ± 0.6	10.75 ± 0.1
		0a	1a	b	b	0c	7c
	5	17.00 ± 1.0	14.72 ± 0.4	14.88 ± 0.54	14.67 ± 0.91	10.86 ± 0.9	5.84 ± 0.54
		0a	8b	b	b	8c	d
	6	17.00 ± 1.0	10.02 ± 1.1			6.10 ± 0.78	5.00 ± 0.76
		0a	5b	8.86 ± 0.13b	8.66 ± 0.33b	c	c
	7	17.00 ± 1.0	6.38 ± 0.70	6.05 ± 0.51c		5.21 ± 0.37	2.95 ± 0.49
		0a	c	d	6.99 ± 0.52b	d	e
ECG	3	60.07 ± 4.9	57.18 ± 5.6	56.61 ± 5.05	54.62 ± 4.56a	51.87 ± 3.4	47.32 ± 1.4
		0a	0ab	ab	b	5b	3b
	4	60.07 ± 4.9	59.48 ± 3.4	55.36 ± 0.67	53.56 ± 0.72	53.63 ± 3.4	49.14 ± 2.8
		0a	0a	ab	b	3b	7b
	5	60.07 ± 4.9	54.74 ± 2.0	50.11 ± 1.90		41.62 ± 1.6	30.44 ± 2.8
		0a	0b	bc	48.80 ± 2.38c	5d	0e
	6	60.07 ± 4.9	46.25 ± 0.7	27.87 ± 1.24		22.55 ± 1.5	22.45 ± 1.2
		0a	8b	c	28.23 ± 2.11c	9d	8d
	7	60.07 ± 4.9	24.13 ± 1.9	18.63 ± 2.87		8.62 ± 0.14	2.35 ± 1.44
		0a	2b	c	16.57 ± 2.77c	d	e
GCG	3	18.57 ± 1.5	17.54 ± 1.2	20.25 ± 1.75	20.52 ± 2.60	23.16 ± 1.2	29.99 ± 2.0
		0c	1c	bc	bc	7b	1a
	4	18.57 ± 1.5	20.95 ± 1.0	24.65 ± 0.93	29.78 ± 1.06	31.13 ± 0.4	37.36 ± 1.7
		0e	9d	c	b	8b	7a
	5	18.57 ± 1.5	32.80 ± 2.1	62.92 ± 3.95	71.46 ± 19.0	106.80 ± 8.	84.54 ± 2.3
		0d	5d	c	7bc	23a	3b
	6	18.57 ± 1.5	80.57 ± 5.0	130.02 ± 3.2	128.32 ± 6.3	115.08 ± 3.	64.15 ± 4.3
		0e	8c	7a	2a	02b	9d
	7	18.57 ± 1.5	99.16 ± 0.9	51.75 ± 2.34	36.66 ± 1.75c	4.21 ± 0.13
		0d	1a	b		e	0.46 ± 0.10f
EGCG	3	279.67 ± 10.50a	258.42 ± 6.81b	253.93 ± 4.98bc	241.35 ± 11.64c	234.89 ± 6.57c	220.07 ± 5.91d
	4	279.67 ± 10	275.76 ± 6.	254.69 ± 6.4	252.71 ± 7.7	231.87 ± 10	181.99 ± 9.
		.50a	30a	0b	5b	.96c	46d
	5	279.67 ± 10	251.99 ± 2.	212.43 ± 1.6	183.97 ± 3.9	147.68 ± 8.	94.91 ± 9.0
		.50a	27b	3c	0d	10e	4f
	6
		279.67 ± 10.50a	186.71 ± 8.11b	117.63 ± 9.57c	109.81 ± 7.07c	91.94 ± 5.44d	59.19 ± 4.85e
	7	279.67 ± 10	81.64 ± 2.6	55.72 ± 4.39	40.22 ± 3.34	8.71 ± 0.94	4.57 ± 0.70
		.50a	0b	c	d	e	e
Catechin s	3	388.43 ± 16	365.52 ± 14	362.33 ± 13.	348.49 ± 14.	341.26 ± 1.	328.30 ± 8.
		.85a	.34b	86bc	32bc	00c	78c
		388.43 ± 16.85a	384.81 ± 8.96ab	362.50 ± 6.47bc	365.71 ± 6.92b	344.30 ± 12.90c	295.20 ± 9.45d
	4
	5	388.43 ± 16	366.23 ± 4.	353.21 ± 5.7	333.40 ± 16.	321.79 ± 2.	228.96 ± 10
		.85a	74b	5b	59c	06c	.75d
	6	388.43 ± 16	337.64 ± 12	298.19 ± 7.6	289.28 ± 10.	248.51 ± 7.	165.42 ± 9.
		.85a	.06b	2c	43c	38d	82e
	7	388.43 ± 16	227.18 ± 5.	146.08 ± 6.3	116.80 ± 4.7	36.36 ± 2.3	15.58 ± 1.6
		.85a	68b	2c	9d	6e	6f

Values are expressed as means ± standard deviations of triplicate measurements.

Different superscript lowercase letters (a, b, c, d) in same row indicate that the difference between the treatments is significantly through LSD test (p < 0.05).