Effects of Microfiltration and Storage Time on Cholesterol, Cis-9, Trans-11 and Trans-10, Cis-12 Conjugated Linoleic Acid Levels, and Fatty Acid Compositions in Pasteurized Milk

Figures & data

TABLE 1 Fat globule size distribution of milk during shelf life

	d32 (μm)			d43 (μm)
	0 d	4 d	7 d	0 d	4 d	7 d
PM	0.50 ± 0.01^Ab	0.48 ± 0.01^Ab	0.48 ± 0.00^Ab	0.70 ± 0.00^Ab	0.70 ± 0.00^Ab	0.69 ± 0.01^Ab
MPM	1.15 ± 0.04^Aa	1.08 ± 0.03^Ba	1.08 ± 0.04^Ba	2.32 ± 0.08^Aa	2.24 ± 0.09^Aa	2.29 ± 0.12^Aa

PM: pasteurized milk; MPM: microfiltered and pasteurized milk; d₃₂: volume-surface average diameter; d₄₃: volumic average diameter; Values were expressed as average ± standard error (SE); Means in same row with same online capital letters were not significantly different (p < 0.05); Means in same column with same online lowercase letters were not significantly different (p < 0.05).

TABLE 2 The contents of total fat, cholesterol, cis-9, trans-11, and trans-10 and cis-12 conjugated linoleic acids (CLAs) of milk during shelf life

	Total fat (g/100 g milk)			Cholesterol (mg/100 g milk)			cis-9, trans-11 CLA (mg/100 g milk)			trans-10, cis-12 CLA (mg/100 g milk)
	0 d	4 d	7 d	0 d	4 d	7 d	0 d	4 d	7 d	0 d	4 d	7 d
PM	3.41 ± 0.01^Aa	3.39 ± 0.03^Aa	3.42 ± 0.02^Aa	12.17 ± 0.15^aB	13.37 ± 0.21^aA	13.57 ± 0.15^aA	16.77 ± 0.59^Ba	17.38 ± 0.27^Ba	18.33 ± 0.11^Aa	0.51 ± 0.02^Ca	0.54 ± 0.00^Ba	0.58 ± 0.01^Aa
MPM	3.42 ± 0.03^Aa	3.42 ± 0.04^Aa	3.32 ± 0.05^Aa	12.30 ± 0.52^aB	13.63 ± 0.21^aA	12.77 ± 0.23^bB	9.56 ± 0.39^Bb	10.08 ± 0.49^ABb	10.52 ± 0.08^Ab	0.51 ± 0.01^Ca	0.53 ± 0.01^Ba	0.56 ± 0.01^Aa

PM: pasteurized milk; MPM: microfiltered and pasteurized milk; Values were expressed as average ± standard error; Means in same row with same online capital letters were not significantly different (p < 0.05); Means in same column with same online lowercase letters were not significantly different (p < 0.05).

TABLE 3 Fatty acid composition of milk during shelf life (mg/100 g milk)

	PM			MPM
Fatty acids	0 d	4 d	7 d	0 d	4 d	7 d
C4:0	66.85 ± 0.77^a	66.17 ± 0.46^ab	61.85 ± 0.80^d	61.54 ± 1.18^d	65.07 ± 0.56^b	63.60 ± 0.13^c
C6:0	50.10 ± 0.30^a	49.16 ± 0.08^b	48.64 ± 0.20^bc	47.29 ± 0.61^d	48.44 ± 0.16^c	47.79 ± 0.49^d
C8:0	32.35 ± 0.04^a	31.75 ± 0.13^b	31.93 ± 0.20^b	30.36 ± 0.24^d	30.83 ± 0.05^c	30.40 ± 0.36^d
C10:0	70.90 ± 0.05^a	69.77 ± 0.38^b	70.28 ± 0.52^ab	65.80 ± 0.31^d	66.69 ± 0.03^c	65.72 ± 0.63^d
C11:0	7.45 ± 0.01^a	7.33 ± 0.01^b	7.36 ± 0.13^ab	7.04 ± 0.01^d	7.18 ± 0.01^c	6.98 ± 0.03^d
C12:0	77.28 ± 0.01^a	76.05 ± 0.42^b	76.77 ± 0.80^ab	71.06 ± 0.06^c	71.48 ± 0.18^c	70.92 ± 0.54^c
C13:0	4.98 ± 0.02^ab	4.85 ± 0.10^c	4.85 ± 0.02^c	5.09 ± 0.06^a	5.02 ± 0.05^ab	4.93 ± 0.08^bc
C14:0	246.33 ± 0.39^a	241.70 ± 1.52^b	243.33 ± 1.00^b	230.39 ± 0.10^c	231.09 ± 0.80^c	229.89 ± 1.83^c
C14:1	18.18 ± 0.06^a	17.87 ± 0.11^b	17.98 ± 0.07^b	17.10 ± 0.01^c	17.13 ± 0.04^c	17.04 ± 0.09^c
C15:0	24.04 ± 0.07^a	23.57 ± 0.15^b	23.73 ± 0.07^b	23.21 ± 0.01^c	23.33 ± 0.08^c	23.29 ± 0.21^c
C16:0	739.25 ± 3.03^b	722.56 ± 4.47^c	728.37 ± 1.35^c	755.77 ± 0.18^a	757.72 ± 2.96^a	755.43 ± 7.41^a
C16:1	40.62 ± 0.29^b	40.24 ± 0.25^b	40.43 ± 0.10^b	41.23 ± 0.01^a	41.36 ± 0.17^a	41.16 ± 0.43^a
C17:0	15.53 ± 0.04^a	15.21 ± 0.11^b	15.31 ± 0.01^b	14.81 ± 0.01^c	14.86 ± 0.04^c	14.78 ± 0.15^c
C18:0	313.09 ± 1.87^a	305.15 ± 2.20^c	308.62 ± 0.24^b	300.75 ± 0.07^d	301.58 ± 0.84^d	300.54 ± 2.32^d
18:1, trans	53.36 ± 0.37^b	54.15 ± 0.29^a	51.39 ± 0.53^c	48.07 ± 0.12^e	49.29 ± 0.01^d	48.49 ± 0.04^e
C18:1n-9	585.14 ± 3.32^a	567.82 ± 4.41c	576.27 ± 0.72^b	573.20 ± 0.39^bc	573.45 ± 2.32^bc	571.56 ± 5.32^bc
18:2, trans	14.16 ± 0.01^c	14.48 ± 0.10^b	14.66 ± 0.08a	13.79 ± 0.06^d	13.83 ± 0.01^d	13.77 ± 0.04d
C18:2n-6, cis	86.33 ± 0.50^a	83.14 ± 0.72^c	84.57 ± 0.65^b	77.72 ± 0.01^d	77.60 ± 0.35^d	77.54 ± 0.74^d
C20:0	3.71 ± 0.02^a	3.61 ± 0.02^c	3.65 ± 0.01^b	3.49 ± 0.01^e	3.53 ± 0.01^d	3.51 ± 0.03^de
C20:1	1.27 ± 0.01^a	1.21 ± 0.01^b	1.18 ± 0.03^cd	1.21 ± 0.02^bc	1.16 ± 0.01^d	1.17 ± 0.02^d
C18:3n-3	9.29 ± 0.07^b	9.06 ± 0.07^c	9.20 ± 0.04^b	9.60 ± 0.02^a	9.62 ± 0.04^a	9.61 ± 0.09^a
C20:2	0.75 ± 0.01^a	0.74 ± 0.01^a	0.74 ± 0.01^a	0.65 ± 0.02^b	0.64 ± 0.01^b	0.65 ± 0.01^b
C22:0	1.76 ± 0.01^a	1.69 ± 0.02^b	1.70 ± 0.01^b	1.57 ± 0.01^c	1.57 ± 0.01^c	1.55 ± 0.03^c
C20:3n-6	5.82 ± 0.03^a	5.70 ± 0.04^b	5.75 ± 0.02^b	5.39 ± 0.03^c	5.40 ± 0.04^c	5.38 ± 0.05^c
C22:1n-9	0.57 ± 0.01^a	0.57 ± 0.01^a	0.56 ± 0.01^a	0.23 ± 0.01^c	0.25 ± 0.01^b	0.25 ± 0.01^b
C20:3n-3	0.17 ± 0.01^b	0.18 ± 0.01^a	0.18 ± 0.01^ab	0.11 ± 0.01^d	0.13 ± 0.01^c	0.13 ± 0.01^c
C20:4n-6	5.36 ± 0.03^a	5.20 ± 0.06^c	5.28 ± 0.01^b	4.87 ± 0.02^d	4.84 ± 0.02^de	4.80 ± 0.03^e
C23:0	1.40 ± 0.01^a	1.37 ± 0.02^b	1.39 ± 0.01^ab	1.20 ± 0.01^e	1.27 ± 0.01^c	1.23 ± 0.02^d
C24:0	1.42 ± 0.02^a	1.39 ± 0.02^b	1.41 ± 0.01^ab	1.26 ± 0.01^c	1.27 ± 0.01^c	1.27 ± 0.02^c
C20:5n-3	0.79 ± 0.02^c	0.76 ± 0.01^c	0.79 ± 0.02^c	0.85 ± 0.02^ab	0.87 ± 0.03^a	0.83 ± 0.02^b
C24:1	0.28 ± 0.01^a	0.28 ± 0.01^a	0.28 ± 0.01^a	0.23 ± 0.01^b	0.23 ± 0.01^b	0.21 ± 0.01^c
C22:6n-3	0.14 ± 0.01^b	0.11 ± 0.01^e	0.12 ± 0.01^d	0.15 ± 0.01^a	0.12 ± 0.01^d	0.13 ± 0.01^c
SFA	1656.45 ± 4.29^a	1621.33 ± 10.09^b	1629.15 ± 5.38^b	1620.64 ± 2.27^b	1630.94 ± 4.05^b	1621.95 ± 13.85^b
MUFA	699.41 ± 4.03^a	682.14 ± 5.05^bc	688.09 ± 0.40^b	681.27 ± 0.49^bc	682.87 ± 2.54^bc	679.88 ± 5.80^c
PUFA	122.81 ± 0.59^a	119.38 ± 0.99^c	121.29 ± 0.61^b	113.13 ± 0.14^d	113.07 ± 0.50^d	112.84 ± 0.97^d
Σn-3	10.39 ± 0.05^b	10.11 ± 0.07^c	10.29 ± 0.06^b	10.71 ± 0.04^a	10.75 ± 0.08^a	10.69 ± 0.11^a
Σn-6	97.51 ± 0.56^a	94.04 ± 0.83^c	95.60 ± 0.63^b	87.98 ± 0.05^d	87.85 ± 0.41^d	87.72 ± 0.82^d
MUFA/SFA	0.422 ± 0.001^ab	0.420 ± 0.000^bc	0.422 ± 0.001^a	0.420 ± 0.001^c	0.419 ± 0.001^cd	0.419 ± 0.000^d
PUFA/SFA	0.0741 ± 0.001^b	0.0736 ± 0.001^c	0.0745 ± 0.001^a	0.0698 ± 0.002^d	0.0693 ± 0.001^e	0.0696 ± 0.000^de
Σn-6/Σn-3	9.39 ± 0.01^a	9.30 ± 0.02^b	9.29 ± 0.01^b	8.22 ± 0.03^c	8.17 ± 0.02^d	8.20 ± 0.01^cd

PM: pasteurized milk; MPM: microfiltered and pasteurized milk; SFA: saturated fatty acids; MUFA: monounsaturated fatty acids; PUFA: polyunsaturated fatty acids; Values were expressed as average ± standard error (SE); Means in same row with same online lowercase letters were not significantly different (p < 0.05).