Multi-Component HPLC Analysis and Antioxidant Activity Characterization of Extracts from Dipsacus sativus (Linn.) Honck

Figures & data

FIGURE 1 Fingerprint chromatogram of the extract from D. sativus obtained from Zhejiang (saponarin was used as the standard reference compound).

TABLE 1 Regression data of the two main components

Peak number	Compounds time (min)	Retention equation^a (R^2)	Regression coefficient (%)	Correlation range (µg)	Content	Linear
2	Saponarin	15.198	y = 2E + 6x + 8629.1	0.9998	38.46	0.4–2.0
3	Isovitexin	16.665	y = 3E + 6x – 42642	0.9999	26.64	0.4–2.0

^ay and x denote the logarithmic values of peak area and concentration, respectively.

TABLE 2 Stability, precision, repeatability, and recovery data of the proposed high-performance liquid chromatography method

Saponarin	Stability	Precision	Repeatability	Recovery
Mean (µg/mL)	80	80	70	40
RSD (%)	2.50	0.43	1.80	0.64

TABLE 3 Effects of D. sativus (Linn.) Honck extract on the contents of SOD and MDA in the blood plasma of mice (mean ± SD)

Group	n	SOD (nmol/mL)	MDA (nmol/mL)
Control	10	276.866 ± 15.692**	12.739 ± 2.406
Model	10	268.142 ± 27.693**	12.374 ± 1.763
D. sativus (Linn.) Honck	15	328.293 ± 21.202	11.551 ± 2.192

The symbol ** represents that differences of same columns are obvious significant (p < 0.01).

FIGURE 2 Structures of main flavonoid components from D. sativus.