Figures & data
Figure 1 RP-TLC of (A) mixture (cholesterol+ß- sitosterol); (B) mixture (cholesterol+ß- sitosterol+ campasterol); (C) cholesterol; (D) ß- sitosterol; (E) USM of pure cow ghee; (F) USM of pure buffalo ghee.
Figure 2 (a) RP-TLC of USM of ghee samples adulterated with adulternt oils: A: 1% groundnut oil adulterated cow ghee; B: 2% groundnut oil adulterated cow ghee; C: 5% groundnut oil adulterated cow ghee; D: 1% sunflower oil adulterated cow ghee; E: 2% sunflower oil adulterated cow ghee; F: 5% sunflower oil adulterated cow ghee; G: 2% soybean oil adulterated cow ghee; H: Pure cow ghee; I: mixture (cholesterol+ß- sitosterol); (b) RP-TLC of USM of ghee samples adulterated with specific adulterant oils: A: mixture (cholesterol+ß-sitosterol); B: Pure cow ghee; C: 1% designer oil adulterated cow ghee; D: 2% designer oil adulterated cow ghee; E: 5% designer oil adulterated cow ghee; F: 10% designer oil adulterated cow ghee; G: 1% designer oil adulterated buffalo ghee; H: 2% designer oil adulterated buffalo ghee; I: 5% designer oil adulterated buffalo ghee; J: 10% designer oil.
Figure 3 RP-TLC of USM of ghee samples adulterated with coconut oil: A: 7.5% coconut oil adulterated cow ghee; B: 10% coconut oil adulterated cow ghee; C: 5% coconut oil adulterated cow ghee; D: pure cow ghee; E: mixture (cholesterol+ß-sitosterol).
Figure 4 (a) HPLC chromatogram of the standard mixture of cholesterol, campasterol, stigmasterol, and β-sitosterol; (b) HPLC chromatogram of USM of pure cow ghee.
Figure 5 (a) HPLC chromatogram of USM of cow ghee adulterated with 2% coconut oil; (b) HPLC chromatogram of USM of cow ghee adulterated with 2% designer oil.
Figure 6 (a) HPLC Chromatogram of USM of cow ghee adulterated with 1% soybean oil; (b) HPLC chromatogram of USM of cow ghee adulterated with 2% sunflower oil; (c) HPLC chromatogram of USM of cow ghee adulterated with 2% groundnut oil.
