High-Performance Liquid Chromatography-Diodic Array Detector, Fungistatic, and Anti-Morphogenical Analysis of Extracts from Psidium brownianum Mart. ex DC. Against Yeasts of the Genus Candida

Figures & data

FIGURE 1 Collection area of Psidium brownianum.

TABLE 1 Phenolics and flavonoids composition of Psidium brownianum

	P. Brownianum			LOD	LOQ
Compounds	EHPB mg/g	AEPBD mg/g	AEPBI mg/g	μg/mL	μg/mL
Gallic acid	3.15 ± 0.02 a	1.73 ± 0.01 a	2.98 ± 0.01 a	0.019	0.062
Catechin	4.28 ± 0.01 b	1.69 ± 0.01 a	1.70 ± 0.03 b	0.008	0.025
Chlorogenic acid	0.09 ± 0.01 c	4.05 ± 0.03 b	3.95 ± 0.02 c	0.024	0.081
Caffeic acid	5.41 ± 0.03 d	1.76 ± 0.01 a	1.68 ± 0.01 b	0.035	0.116
Ellagic acid	4.35 ± 0.01 b	3.84 ± 0.02 b	1.62 ± 0.01 b	0.010	0.034
Rutin	2.67 ± 0.01 e	4.29 ± 0.01 c	2.89 ± 0.03 a	0.017	0.056
Quercitrin	5.63 ± 0.02 f	4.32 ± 0.01 c	3.11 ± 0.01 a	0.032	0.105
Quercetin	11.54 ± 0.02 g	7.08 ± 0.03 d	4.05 ± 0.02 c	0.025	0.083
Coumarin	7.18 ± 0.02 h	1.81 ± 0.02 a	3.10 ± 0.03 a	0.011	0.037
Kaempferol	8.93 ± 0.01 i	6.97 ± 0.01 d	6.92 ± 0.01 d	0.018	0.059
Luteolin	5.61 ± 0.01 f	10.34 ± 0.02 e	8.37 ± 0.01 e	0.023	0.075

Results are expressed as mean ± standard deviation of the determinations; Averages followed by different letters on each extract differ by Tukey test at p < 0.05; AEPBI: Aquous extract of P. brownianum infusion; AEPBD: Aquous extract of P. bronianum decoction; EHPB: Hidroethanolic extract of P. brownianum.

FIGURE 2 High-performance liquid chromatography phenolics and flavonoids profile of Psidium brownianum.

TABLE 2 IC₅₀ of all products assayed (µg/mL)

Yeast strains	EHPB	AEPBD	AEPBI	Fluconazole
CA INCQS 40006	1056.82	2924.15	4073.8	76.72
CA LM 62	1840.77	3872.58	4764.31	81.35
CA LM 77	1199.5	3090.3	4168.69	69.09
CA LM 109	1396.37	3689.78	4602.57	72.49
CA LM 111	1862.09	2460.37	4677.35	103.4
CA LM 122	1076.47	3162.28	4677.35	103.4
CT INCQS 40042	1333.52	3589.22	5011.87	73.98
CT LM 18	1651.96	3890.45	4943.11	391.2
CT LM 20	1815.52	3630.78	5128.61	205.5
CT LM 23	1883.65	3597.49	4655.86	58.63

AEPBD: Aquous extract of P. brownianum infusion; EHPB: Aquous extract of P. bronianum decoction; Hidroethanolic extract of P. brownianum. The indicated strains in bold were utilized in the assays.

FIGURE 3 Cell viability curve Candida strains under the effect of Psidium brownianum.

FIGURE 4 Controls used in micromorphologic assay of Candida yeast under the effect of the products of Psidium brownianum.

FIGURE 5 Effect of Psidium brownianum extracts on the morphology of Candida albicans.

FIGURE 6 Effect of Psidium brownianum extracts on the morphology of Candida tropicalis.