Use of High-Performance Size-Exclusion Chromatography for Characterization of Amylose Isolated from Diverse Botanical Sources

Figures & data

TABLE 1 Amylose content and λ_max of amyloses from diverse botanical sources

Sample	*Amylose (%)	λmax (nm)
Commercial	85.6 ± 0.019^a	610
Potato	92.6 ± 0.003^d	601
Mango	88.5 ± 0.003^b	605
Maize	90.7 ± 0.008^c	610
Apple	86.0 ± 0.007^a	607

*Values are mean of triplicate determinations ± standard error; Different lowercase letters in the same column show significant difference (p < 0.05).

FIGURE 1 Semi-logarithmic plot of molar mass versus elution volume for pullulan standards.

TABLE 2 Number-average (Mn) and weight-average (Mw) molar masses of starches amyloses*

Sample	Solubility (%)	Mn (10^–5 g/mol)	Mw (10^–5 g/mol)	Mw/Mn
Commercial	99.73 ± 0.15^b	1.50 ± 0.10^a	2.00 ± 0.10^b	2.70 ± 0.01^c
Potato	98.96 ± 1.70^b	2.20 ± 0.20^b	3.00 ± 0.20^c	4.10 ± 0.05^e
Mango	87.50 ± 2.80^a	1.40 ± 0.10^a	1.90 ± 0.05^b	1.30 ± 0.04^a
Maize	85.00 ± 1.41^a	8.80 ± 0.20^d	1.20 ± 0.01^a	1.40 ± 0.02^b
Apple	99.70 ± 0.42^b	5.70 ± 0.40^c	8.50 ± 0.40^d	2.90 ± 0.09^d

*Values are mean of triplicate determination ± standard error; Different lowercase letters in the same column show significant difference (p < 0.05).

FIGURE 2 Chromatograms of amyloses from diverse botanical source.