Study on Antioxidant Activity and Amino Acid Analysis of Rapeseed Protein Hydrolysates

Figures & data

FIGURE 1 Gel chromatography of the purification of rapeseed protein hydrolysates by the Sephacryl S-100HR column.

FIGURE 2 The antioxidant activity of the groups of RPHs; A: ORAC values of the groups of RPHs; B: PSC values of the groups of RPHs; C: Cytotoxic effects of the groups of RPHs by using MTT dye; D: CAA values of the groups of RPHs. Note: Histograms marked with different letters are significant at p < 0.05.

FIGURE 3 Cellular antioxidant capacity EC₅₀ values. Note: Histograms marked with different letters are significant at p < 0.05.

TABLE 1 Amino acid compositions of group 3

Essential amino acid		Non-essential amino acids
Types of amino acids	Amino acid content/(g/100 g)	Types of amino acids	Amino acid content/(g/100 g)
Thr	0.412	Asp	0.611
Val	0.366	Ser	0.473
Met	2.963	Glu	18.875
Ile	3.07	Gly	6.482
Leu	0.647	Ala	6.245
Phe	0.593	Cys	0.092
Lys	0.632	Tyr	7.901
His	0.308	Pro	3.132
Arg	0.777
Total quantity	9.768	Total quantity	43.811
Total amino acid ratio%	18.23	Total amino acid ratio%	81.77

FIGURE 4 The antioxidant activity of the groups separated by RP–HPLC; A: ORAC values of each group separated by RP–HPLC; B: PSC values of each group separated by RP–HPLC.

FIGURE 5 Separation of fraction 5 on RP–HPLC.

FIGURE 6 MS1 spectrum of group 5.

FIGURE 7 A: ESI–MS/MS spectrum of group 5 (m/z < 200); B: ESI–MS/MS spectrum of group 5 (m/z > 200).