Amino acid decarboxylase activities and biogenic amine formation abilities of lactic acid bacteria isolated from shalgam

Figures & data

Table 1. Amino acid decarboxylase activity test results of lactic acid bacteria isolated from shalgam.

	Amino acid decarboxylase activity*
LAB isolates	Arg	Tyr	Orn	Lys	Trp	His	Phe
Lactobacillus spp. (48 isolates)	+	+	–	–	–	–	–
Lactobacillus sp. 2C5	+	–	–	–	–	–	–
Lactobacillus sp. 4C10	–	+	–	–	–	–	–
Lactobacillus sp. 0A8	+	+	–	–	–	–	–
Lactococcus spp. (3 isolates)	+	+	–	–	–	–	–
Leuconostoc sp. 4E9	–	+	–	–	–	–	–
Streptococcus sp. 4E7	+	+	–	–	–	–	–

*Arg: arginine; Tyr: tyrosine; Orn: ornithine; Lys: lysine; Trp: tyrptophane; His: histidine.

Table 2. Putrescine, agmatine and tyramine amounts produced by LAB isolates in MRS broth.

LAB isolates	Biogenic amine levels (mg L^–1)
	Putrescine	Agmatine	Tyramine
Lactobacillus spp. (48 isolates)	2.1 ± 0.6 – 33.3 ± 1.7	105.8 ± 14.0 – 867.5 ± 21.9	24.5 ± 6.9 – 649.7 ± 55.1
Lactobacillus sp. 2C5 ^(1)	6.0 ± 1.5	446.8 ± 74.3	225.7 ± 76.4
Lactobacillus sp. 4C10 ^(2)	4.1 ± 0.9	338.0 ± 75.3	161.1 ± 19.9
Lactobacillus sp. 0A8	ND^(3)	154.6 ± 12.2	97.4 ± 4.3
Lactococcus spp. (3 isolates)	5.2 ± 1.6 – 10.0 ± 1.7	219.3 ± 74.9 – 371.0 ± 22.0	271.2 ± 30.0 – 355.9 ± 26.7
Leuconostoc sp. 4E9 ^(2)	3.9 ± 0.2	152.3 ± 43.7	158.9 ± 23.1
Streptococcus sp. 4E7	4.7 ± 0.5	205.6 ± 17.4	111.9 ± 31.5

⁽¹⁾Tyrosine decarboxylase negative.

⁽²⁾Arginine decarboxylase negative.

⁽³⁾Not detected.

Figure 1. Chromatograms of biogenic amine standards (A) and a LAB isolate (B)