Laundry detergent-stable lipase from Pacific white shrimp (Litopenaeus vannamei) hepatopancreas: Effect of extraction media and biochemical characterization

Figures & data

Table 1. Effect of extraction media on the recovery of lipase from Pacific white shrimp hepatopancreas*.

Extraction media	Total activity (Units)	Total protein (mg)	Specific activity (Units/mg Protein)**
Distilled water	65576 ± 1215^a	2003 ± 24^b	32.76 ± 0.61^a
50 mM Tris-HCl, pH 7	71459 ± 2096^b	1728 ± 62^a	41.35 ± 1.21^c
50 mM Na-Phosphate, pH 7	65468 ± 3907^a	1746 ± 33^a	37.51 ± 2.24^b

*The defatted Pacific white shrimp hepatopancreas powder was extracted in different media at 4°C for 30 min and hydrolytic activity was analyzed using p-nitrophenyl palmitate as substrate at pH 7.5 and 37°C.

**Mean ± SD from triplicate determinations.

The different letters in the same column denote the significant difference (p < 0.05).

Figure 1. Effect of some surfactants on total activity (a) and specific activity (b) of lipase from Pacific white shrimp hepatopancreas. Different letters indicate significances (p < 0.05). Bars represent the standard deviation from triplicate determinations.

Figure 2. pH (a) and temperature (b) profiles of lipase from hepatopancreas of Pacific white shrimp. Bars represent the standard deviation from triplicate determinations.

Figure 3. Thermal (a) and pH (b) stability of lipase from hepatopancreas of Pacific white shrimp. Bars represent the standard deviation from triplicate determinations.

Figure 4. Effect of NaDC concentration of lipase from hepatopancreas of Pacific white shrimp. Bars represent the standard deviation from triplicate determinations.

Figure 5. Effect of NaCl concentration of lipase from hepatopancreas of Pacific white shrimp. Bars represent the standard deviation from triplicate determinations.

Table 2. Effect of selected chemicals on the stability of lipase from hepatopancreas of Pacific white shrimp.

Substance	Concentration	Relative activity (%)^*
Control	0	100.00 ± 0.17^h
MgCl2	1 mM	88.50 ± 0.61^fg
	10 mM	84.78 ± 5.67^ef
CaCl2	1 mM	92.08 ± 1.83^g
	10 mM	89.70 ± 0.98^fg
MnCl2	1 mM	81.02 ± 1.08^de
	10 mM	78.81 ± 0.96^de
HgCl2	1 mM	83.37 ± 1.45^ef
	10 mM	22.99 ± 1.24^b
CuCl2	1 mM	48.12 ± 3.40^c
	10 mM	13.41 ± 0.40^a
AlCl3	1 mM	84.67 ± 2.77^ef
	10 mM	15.22 ± 11.87^a
NaN3	1 mM	102.46 ± 5.28^h
	10 mM	117.79 ± 4.04^i
EDTA	1 mM	99.17 ± 4.38^h
	10 mM	74.48 ± 0.89^d
PMSF	1 mM	52.86 ± 4.85^c
	10 mM	27.30 ± 1.24^b

*Mean ± SD from triplicate determinations.

The different letters in the same column denote the significant difference (p < 0.05).

Table 3. Effect of different surfactants on the stability of lipase from Pacific white shrimp hepatopancreas.

Surfactants	Concentration (%)	Relative activity (%)*
Control	0	100.00 ± 3.61^f
Tween 20	1	100.07 ± 7.84^f
	10	80.95 ± 0.09^d
Tween 80	1	87.45 ± 6.38^e
	10	72.42 ± 0.11^c
Triton X-100	1	86.80 ± 1.14^e
	10	60.81 ± 2.51^b
SDS	1	76.65 ± 1.73^cd
	10	39.91 ± 1.04^a
Gum arabic	1	100.92 ± 1.22^f
	10	91.92 ± 0.43^e

*Mean ± SD from triplicate determinations.

The different letters denote the significant difference (p < 0.05).

Figure 6. Stability of crude lipase from hepatopancreas of Pacific white shrimp in presence of various commercial liquid (a) and solid (b) laundry detergents. The different letters indicate significant differences (p < 0.05).