Isolation and 2,2’-diphenyl-1-picrylhydrazyl radical scavenging activity of active compound from Jujube tree (Zizyphus mauritiana Auct. non Lamk.)

Figures & data

Table 1. The activity of DPPH free radical scavenging activity of methanol extract and fractions of the Jujube tree bark.

Samples	IC50 (µg/mL)
Methanol extract	26.83
Trituration of methanol extract:
N-hexan fraction	310.48
Ethyl acetate fraction	23.70
Residual methanol extract	40.71
Fractination of ethyl acetate fraction:
E1 fraction	214.17
E2 fraction	231.53
E3 fraction	355.01
E4 fraction	17.60
E5 fraction	32.23

Table 2. The total of phenolic and flavonoid content of methanol extract and fractions.

Samples	Total of phenolic content (% b/b GAE)	Total of flavonoid content (% b/b QE)
Methanol extract	11.01 ± 0.16	9.07 ± 0.42
Trituration of methanol extract:
N-Hexan fraction	—	—
Ethyl acetate fraction	21.20 ± 0.10	17.92 ± 0.35
Residual methanol extract	36.29 ± 0.10	15.69 ± 0.33
Fractination of ethyl acetate fraction:
E1 fraction	5.41 ± 0.19	—
E2 fraction	5.85 ± 0.13	—
E3 fraction	12.64 ± 0.16	5.73 ± 0.255
E4 fraction	22.83 ± 0.17	21.31 ± 0.26
E5 fraction	29.87 ± 0.17	8.29 ± 0.42

Note: % GAE: gallic acid equivalent; % QE: quercetin equivalent.

Figure 1. The relation between the total of phenolic content expressed as gallic acid equivalent with IC_50.

Figure 2. Relation between the total flavonoid content expressed as % quercetin equivalent (% wt/wt) with IC₅₀.