β-N-oxalyl-L-2,3-diaminopropionic acid, L-homoarginine, and asparagine contents in the seeds of different genotypes Lathyrus sativus L. as determined by UHPLC-MS/MS

Figures & data

Table 1. Transitions, collisions energies, retention times of β-ODAP, L-homoarginine, and asparagine.

	Parent (m/z)	Transition Ion1 (m/z)	Transition Ion2 (m/z)	Collision Energy (V)	Retention Times (min)
β-ODAP	177.2	116.2	88.3	15	0.98
L-homoarginine	189.0	84.1	144.1	20	0.84
Asparagine	133.1	74.1	87.2	15	0.86

Table 2. Recovery (%), RSD (%), and LOQ (mg/L) of β-ODAP, L-homoarginine, and asparagine.

	%Recovery (n = 6)	%RSD	LOQ (mg/L)
β-ODAP	84.62	9.61	1.51
L-homoarginine	92.35	3.25	0.41
Asparagine	93.52	3.66	0.45

RSD: relative standard deviation of six samples and LOQ: limit of quantification.

Figure 1. Chromatograms of β-ODAP, asparagine, and L-homoarginine.

Table 3. Contents of β-ODAP, L-homoarginine, and asparagine of 173 grass pea genotypes.

Genotypes	β-ODAP(mg/g)	L-homoarginine (mg/g)	Asparagine (mg/g)	Genotypes	β-ODAP(mg/g)	L-homoarginine (mg/g)	Asparagine (mg/g)	Genotypes	β-ODAP(mg/g)	L-homoarginine (mg/g)	Asparagine (mg/g)
LS-1	4.87	8.61	1.95	LS-59	4.51	7.82	2.08	LS-117	3.69	9.17	3.92
LS-2	5.38	3.02	2.25	LS-60	5.33	7.78	3.76	LS-118	3.19	6.40	1.76
LS-3	5.05	4.55	1.62	LS-61	5.74	4.95	3.28	LS-119	3.92	6.22	3.35
LS-4	4.90	3.36	1.65	LS-62	5.11	5.02	2.11	LS-120	4.03	4.03	3.43
LS-5	8.16	4.67	1.76	LS-63	5.00	6.52	2.95	LS-121	3.88	7.63	3.70
LS-6	5.81	6.56	4.04	LS-64	4.50	5.46	2.65	LS-122	2.67	5.46	3.29
LS-7	6.62	7.07	2.71	LS-65	5.62	3.72	2.15	LS-123	3.81	4.52	1.60
LS-8	5.08	4.05	2.07	LS-66	6.14	5.32	3.59	LS-124	3.16	3.63	0.76
LS-9	5.32	3.02	1.12	LS-67	5.76	3.28	2.81	LS-125	3.90	6.74	2.74
LS-10	6.54	4.80	2.36	LS-68	7.19	8.00	3.97	LS-126	4.11	7.54	2.24
LS-11	5.57	7.39	3.18	LS-69	5.10	5.16	1.82	LS-127	3.70	5.60	2.17
LS-12	6.56	3.92	1.84	LS-70	7.65	5.26	2.17	LS-128	4.19	6.46	3.08
LS-13	6.15	6.85	2.42	LS-71	5.98	6.61	2.56	LS-129	3.68	10.86	2.49
LS-14	5.48	3.98	2.13	LS-72	6.05	8.49	2.61	LS-130	4.32	6.15	3.33
LS-15	5.53	4.27	2.63	LS-73	8.07	3.89	2.46	LS-131	4.14	7.06	2.41
LS-16	5.73	3.85	2.33	LS-74	6.26	1.45	1.69	LS-132	3.85	5.81	2.73
LS-17	6.06	6.01	2.50	LS-75	4.60	1.80	1.56	LS-133	4.02	6.49	2.52
LS-18	6.32	3.10	2.26	LS-76	4.73	2.20	4.21	LS-134	5.48	7.87	2.43
LS-19	7.58	5.04	3.48	LS-77	4.89	1.82	1.90	LS-135	5.24	11.22	2.70
LS-20	5.76	5.56	2.04	LS-78	5.10	4.05	2.89	LS-136	3.60	6.58	2.81
LS-21	5.94	4.57	2.38	LS-79	5.66	5.77	2.65	LS-137	4.43	6.53	2.22
LS-22	5.97	4.14	2.58	LS-80	5.07	6.74	2.33	LS-138	4.42	7.47	4.02
LS-23	7.27	3.88	2.97	LS-81	5.30	5.49	2.63	LS-139	4.67	5.87	3.34
LS-24	5.84	5.21	2.93	LS-82	4.71	1.66	1.88	LS-140	3.43	6.49	3.51
LS-25	5.08	5.74	3.32	LS-83	5.94	1.63	1.42	LS-141	3.03	5.55	2.39
LS-26	7.46	7.30	3.61	LS-84	5.66	4.51	3.06	LS-142	3.05	3.48	3.77
LS-27	5.38	7.03	2.77	LS-85	6.45	6.79	3.01	LS-143	4.25	7.15	5.02
LS-28	6.56	5.70	3.08	LS-86	5.98	2.23	2.12	LS-144	3.98	5.90	5.08
LS-29	4.18	6.75	3.70	LS-87	5.24	7.54	3.49	LS-145	4.36	4.81	2.71
LS-30	7.14	8.60	2.77	LS-88	5.55	5.82	2.73	LS-146	6.16	5.81	4.27
LS-31	5.45	5.06	2.61	LS-89	3.31	6.52	1.42	LS-147	6.93	11.64	4.57
LS-32	5.91	7.10	3.36	LS-90	4.63	6.39	3.77	LS-148	6.69	6.74	4.65
LS-33	7.28	4.06	3.71	LS-91	3.69	9.58	3.44	LS-149	8.09	6.49	4.17
LS-34	7.77	3.31	2.34	LS-92	3.86	7.28	3.00	LS-150	4.69	7.09	3.12
LS-35	6.31	7.66	3.35	LS-93	4.26	9.28	2.23	LS-151	4.73	9.14	5.22
LS-36	7.31	5.38	2.43	LS-94	4.10	4.72	2.63	LS-152	3.27	6.77	3.25
LS-37	8.20	5.22	2.54	LS-95	3.89	4.94	2.50	LS-153	3.16	5.44	2.63
LS-38	7.99	5.57	2.42	LS-96	3.32	8.21	3.87	LS-154	4.27	4.53	3.46
LS-39	8.02	3.48	2.20	LS-97	4.52	2.54	0.59	LS-155	4.33	7.15	2.83
LS-40	5.88	7.05	2.75	LS-98	3.88	3.12	2.70	LS-156	3.29	7.10	3.66
LS-41	6.25	6.43	4.02	LS-99	5.04	6.99	2.89	LS-157	3.76	4.76	3.15
LS-42	6.14	4.45	2.50	LS-100	3.83	3.78	3.04	LS-158	3.78	5.30	4.56
LS-43	5.65	5.16	3.16	LS-101	3.95	7.96	2.42	LS-159	3.74	3.01	2.18
LS-44	5.38	4.77	3.21	LS-102	3.81	4.88	1.89	LS-160	4.22	8.51	2.25
LS-45	6.71	4.95	2.33	LS-103	4.08	5.09	3.30	LS-161	4.39	8.81	1.17
LS-46	4.15	3.34	2.73	LS-104	5.17	4.38	2.78	LS-162	5.37	6.45	2.72
LS-47	5.42	5.01	2.93	LS-105	2.84	4.61	4.52	LS-163	4.23	8.41	2.20
LS-48	5.08	4.06	3.13	LS-106	1.55	10.83	2.43	LS-164	3.64	3.49	2.95
LS-49	5.49	1.35	1.35	LS-107	5.19	4.45	2.34	LS-165	2.24	8.65	3.23
LS-50	5.76	6.32	2.10	LS-108	4.77	2.27	2.07	LS-166	2.93	8.16	2.81
LS-51	6.73	7.64	3.59	LS-109	4.97	5.25	2.80	LS-167	4.68	6.25	4.24
LS-52	6.53	6.43	1.78	LS-110	3.76	5.84	3.60	LS-168	6.57	6.86	2.49
LS-53	8.00	8.66	2.56	LS-111	4.45	3.54	4.65	LS-169	3.04	6.94	0.85
LS-54	6.59	6.52	2.77	LS-112	6.32	4.26	2.29	Ceora	3.52	4.17	2.81
LS-55	5.41	4.85	3.50	LS-113	3.41	4.79	3.34	Eren	2.81	6.42	2.14
LS-56	4.66	3.23	1.75	LS-114	4.01	3.16	2.29	İptaş	5.32	3.30	2.35
LS-57	5.64	4.21	1.95	LS-115	2.76	4.76	2.87	Karadağ	3.21	2.83	2.82
LS-58	4.86	5.80	2.33	LS-116	3.80	5.88	3.68

Figure 2. Histogram of β-ODAP L-homoarginine and asparagine with normal curve, and normal probability plot for β-ODAP L-homoarginine and asparagine.

Figure 3. The best linear and disperse graphics β-ODAP between L-homoarginine and asparagine.