Structural and functional properties of Maillard reaction products of protein isolate (mung bean, Vigna radiate (L.)) with dextran

Figures & data

Figure 1. Effects of reaction time on DG and brown degree of MBPI–Dextran conjugates obtained at 80°C (A) and 90°C (B).

Figure 2. Effects of reaction time on solubility of MBPI–Dextran conjugates obtained at 80°C (A) and 90°C (B).

Figure 3. Effects of reaction time on emulsifying properties of MBPI–Dextran conjugates obtained at 80°C (A) and 90°C (B).

Figure 4. Effects of reaction time on surface hydrophobicity of MBPI–Dextran conjugates obtained at 80°C (A) and 90°C (B).

Figure 5. SDS-PAGE patterns of MBPI and MBPI–Dextran conjugates obtained at 80°C (A) and 90°C (B). (Lane M: Protein standards; Lane 1: native mung bean protein isolates; Lane 2: mixture of mung bean protein isolates and dextran; Lane 3–8: MBPI-D conjugates incubated under 1–6 h).

Figure 6. Fluorescence spectra of MBPI and MBPI–D conjugates obtained at 80°C (A) and 90°C (B).

Table 1. Secondary structural content of native MBPI and MBPI–Dextran conjugates treated at 80°C estimated from circular dichroism spectra.

Sample	α-Helix (%)	β-Sheet (%)	β-Turn (%)	Unordered structure (%)
MBPI	19.7 ± 0.1^g*	26.7 ± 0.1^a	21.3 ± 0.2^a	32.3 ± 0.1^c
MBPI-D mixture	19.4 ± 0.1^f	26.6 ± 0.1^a	21.3 ± 0.0^a	32.7 ± 0.1^d
MBPI-D-80-1**	8.8 ± 0.1^a	35.6 ± 0.1^e	23.5 ± 0.2^f	32.1 ± 0.1^b
MBPI-D-80-2	10.4 ± 0.0^b	34.3 ± 0.1^d	22.6 ± 0.0^c	32.7 ± 0.2^d
MBPI-D-80-3	12.2 ± 0.0^e	31.6 ± 0.0^b	21.7 ± 0.1^b	34.6 ± 0.0^f
MBPI-D-80-4	11.0 ± 0.1^c	33.3 ± 0.1^d	22.8 ± 0.1^d	33.0 ± 0.0^e
MBPI-D-80-5	11.4 ± 0.2^d	32.6 ± 0.2^c	23.3 ± 0.0^ef	32.8 ± 0.1^d
MBPI-D-80-6	11.4 ± 0.1^d	33.4 ± 0.1^d	23.3 ± 0.2^ef	31.8 ± 0.0^a

*Means with the different letters within a column are significant difference (P < 0.05).

**Means different grafted time.

Table 2. Secondary structural content of native and MBPI–Dextran conjugates treated at 90°C estimated from circular dichroism spectra.

Sample	α-Helix (%)	β-Sheet (%)	β-Turn (%)	Unordered structure (%)
MBPI	19.7 ± 0.1^f*	26.7 ± 0.1^e	21.3 ± 0.1^b	32.3 ± 0.1^b
MBPI-D-90	19.4 ± 0.1^e	26.6 ± 0.1^f	21.3 ± 0.0^d	32.7 ± 0.1^a
MBPI-D-90-1**	12.3 ± 0.2^d	33.1 ± 0.1^b	22.7 ± 0.2^c	31.9 ± 0.1^d
MBPI-D-90-2	14.5 ± 0.1^f	29.9 ± 0.2^a	22.6 ± 0.0^b	32.9 ± 0.0^g
MBPI-D-90-3	12.1 ± 0.0^c	33.1 ± 0.0^b	23.3 ± 0.1^e	31.6 ± 0.2^c
MBPI-D-90-4	11.4 ± 0.1^b	33.2 ± 0.0^b	23.0 ± 0.1^d	32.4 ± 0.0^e
MBPI-D-90-5	11.4 ± 0.1^b	33.5 ± 0.1^c	22.5 ± 0.0^bc	32.7 ± 0.1^f
MBPI-D-90-6	10.7 ± 0.1^a	34.1 ± 0.1^d	21.1 ± 0.1^a	34.0 ± 0.1^h

*Means with the different letter within a column are significantly different (P < 0.05).

**Means different grafted time.

Figure 7. Far-UV CD spectra of native and MBPI–Dextran conjugates treated at 80°C (A) and 90°C (B).