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Original Articles

Formulation and characterization of oil-in-water emulsions stabilized by gelatinized kudzu starch

, ORCID Icon, , ORCID Icon, ORCID Icon & ORCID Icon
Pages 1329-1341 | Received 15 Nov 2016, Accepted 23 Jun 2017, Published online: 14 Dec 2017

Figures & data

Figure 1. Effects of gelatinization conditions of kudzu starch on the interfacial tension. (a) Effect of gelatinization time on interfacial tension between soybean oil and gelatinized kudzu starch dispersion. (b) Effect of gelatinization temperature on interfacial tension between soybean oil and gelatinized kudzu starch dispersion. The different letter shows significant difference at the 95% probability level.

Figure 1. Effects of gelatinization conditions of kudzu starch on the interfacial tension. (a) Effect of gelatinization time on interfacial tension between soybean oil and gelatinized kudzu starch dispersion. (b) Effect of gelatinization temperature on interfacial tension between soybean oil and gelatinized kudzu starch dispersion. The different letter shows significant difference at the 95% probability level.

Figure 2. The particle size distribution of native kudzu starch and gelatinized kudzu starch. (

) denotes the distribution of particle size of native kudzu starch, (
) denotes the distribution of particle size of gelatinized kudzu starch, and (
) denotes the distribution of particle size of gelatinized kudzu starch stored for 1 month.

Figure 2. The particle size distribution of native kudzu starch and gelatinized kudzu starch. (Display full size) denotes the distribution of particle size of native kudzu starch, (Display full size) denotes the distribution of particle size of gelatinized kudzu starch, and (Display full size) denotes the distribution of particle size of gelatinized kudzu starch stored for 1 month.

Figure 3. Microstructure of O/W emulsions. (a) Microstructure of freshly prepared O/W emulsions stabilized with gelatinized starch. (i) Formulated with rotor-stator homogenizer and (ii) formulated with high-pressure homogenizer. (b) Confocal laser scanning microscopy image of O/W emulsions stabilized by gelatinized kudzu starch dispersion.

Figure 3. Microstructure of O/W emulsions. (a) Microstructure of freshly prepared O/W emulsions stabilized with gelatinized starch. (i) Formulated with rotor-stator homogenizer and (ii) formulated with high-pressure homogenizer. (b) Confocal laser scanning microscopy image of O/W emulsions stabilized by gelatinized kudzu starch dispersion.

Figure 4. Storage stability of O/W emulsions stabilized using gelatinized starch. (a) d3,2 of O/W emulsions formulated with rotor-stator homogenizer and/or high-pressure homogenizer. (

) denotes emulsions performed with rotor-stator homogenizer and (
) denotes emulsions performed with high-pressure homogenizer. (b) O/W emulsion picture (left) formulated by rotor-stator homogenizer at days 0 and 9 and O/W emulsion picture formulated by high-pressure homogenizer (right) at days 0 and 9. The different letter shows significant difference at the 95% probability level.

Figure 4. Storage stability of O/W emulsions stabilized using gelatinized starch. (a) d3,2 of O/W emulsions formulated with rotor-stator homogenizer and/or high-pressure homogenizer. (Display full size) denotes emulsions performed with rotor-stator homogenizer and (Display full size) denotes emulsions performed with high-pressure homogenizer. (b) O/W emulsion picture (left) formulated by rotor-stator homogenizer at days 0 and 9 and O/W emulsion picture formulated by high-pressure homogenizer (right) at days 0 and 9. The different letter shows significant difference at the 95% probability level.

Figure 5. (a) The d3,2 of O/W emulsions containing different kudzu starch concentrations. (

) denotes 0% (w/w), (
) denotes 0.1% (w/w), (
) denotes 1% (w/w), (
) denotes 3% (w/w), and (
) denotes 5% (w/w). (b) Viscosities of gelatinized kudzu starch dispersion with different concentrations. The different letter shows significant difference at the 95% probability level.

Figure 5. (a) The d3,2 of O/W emulsions containing different kudzu starch concentrations. (Display full size) denotes 0% (w/w), (Display full size) denotes 0.1% (w/w), (Display full size) denotes 1% (w/w), (Display full size) denotes 3% (w/w), and (Display full size) denotes 5% (w/w). (b) Viscosities of gelatinized kudzu starch dispersion with different concentrations. The different letter shows significant difference at the 95% probability level.

Table 1. The pictures of O/W emulsions containing different kudzu starch concentrations stored in test tubes at 25°C at days 0 and 9.

Table 2. Physicochemical properties of dispersed phases used in the present study.

Figure 6. The d3,2 of O/W emulsions containing different oil types. The emulsions contained 5% (w/w) oil in the dispersed phase. (

) denotes soybean oil, (
) denotes MCT, and (
) denotes limonene.

Figure 6. The d3,2 of O/W emulsions containing different oil types. The emulsions contained 5% (w/w) oil in the dispersed phase. (Display full size) denotes soybean oil, (Display full size) denotes MCT, and (Display full size) denotes limonene.

Figure 7. The d3,2 of O/W emulsions containing different soybean oil weight fractions. (

) denotes 5% (w/w), (
) denotes 10% (w/w), (
) denotes 20% (w/w), and (
) denotes 30% (w/w). The different letter shows significant difference at the 95% probability level.

Figure 7. The d3,2 of O/W emulsions containing different soybean oil weight fractions. (Display full size) denotes 5% (w/w), (Display full size) denotes 10% (w/w), (Display full size) denotes 20% (w/w), and (Display full size) denotes 30% (w/w). The different letter shows significant difference at the 95% probability level.

Tabel 3. The micrographs of O/W emulsions together with pictures.

Figure 8. The microstructures of O/W emulsions kept at 25°C for 24 h stabilized by different gelatinized starches together with pictures. The oil phase was stained with Sudan IV dye, and the continuous phase was dyed with methylene blue for the observation of emulsions. (1) Emulsions stabilized with kudzu starch, (2) rice starch, (3) wheat starch, and (4) corn starch.

Figure 8. The microstructures of O/W emulsions kept at 25°C for 24 h stabilized by different gelatinized starches together with pictures. The oil phase was stained with Sudan IV dye, and the continuous phase was dyed with methylene blue for the observation of emulsions. (1) Emulsions stabilized with kudzu starch, (2) rice starch, (3) wheat starch, and (4) corn starch.

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