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Figures & data
Figure 1. Schematic of the procedures for the stability assay. For more details on the terminology used to designate the fractions, see the Materials and Methods section. LC-MS/ESI: Liquid chromatography coupled with mass spectrometry/electron spray ionization analysis; DPPH: 1,1-diphenyl-2-picryl-hydrazyl; ·NO: nitric oxide; O2·–: anion superoxide; and ·OH: hydroxyl capture assays. Total phenolic determinations were performed at least five times, on different days, in triplicate for each sample. Determinations of antioxidant activity (evaluated using DPPH, the reducing power test, and •NO, •OH, and O2·– capture assays) were performed at least twice, in triplicate, for each concentration of each sample tested.
Table 1. Polyphenol compounds of avocado leaf extract identified by LC-MS/ESI.
Figure 2. LC-MS/ESI chromatogram of avocado leaf extract. Numbers in the chromatogram refer to the compounds described in . For information of the MS spectra, see and Fig. S1.
Table 2. EC50 values for the PA extract and standards calculated for DPPH, •NO, •OH, and O2•– capture assays.
Figure 3. Characteristics of avocado leaf extract derived from leaves subjected to different drying conditions. Avocado leaf extracts were analysed by measuring the total phenolic content (A), DPPH radical capture test (B), and reducing power test (C). The resulting values are expressed as a mean ± standard deviation calculated according to each method. In (A), different letters mean significant differences as determined using Tukey’s test (*p ≤ 0.05). Results from Pearson correlation analyses (D and E) are also provided.
Table 3. DPPH EC50 values of the fractions obtaining from stability assays of avocado leaf extract.
