Figures & data
Figure 9. The thawing performance of the different freezing rates: (a) 0.5°C/min, (b) 1.2°C/min, and (c) 2°C/min.
![Figure 9. The thawing performance of the different freezing rates: (a) 0.5°C/min, (b) 1.2°C/min, and (c) 2°C/min.](/cms/asset/8350534d-23e0-4543-97f0-1c0817b1350a/ljfp_a_1439506_f0009_oc.jpg)
Figure 10. Cell plasmolysis after thawing at the different freezing rates: (a) 2°C/min, (b) 1.2°C/min, (c) 0.5°C/min, and (d) 0.1°C/min.
![Figure 10. Cell plasmolysis after thawing at the different freezing rates: (a) 2°C/min, (b) 1.2°C/min, (c) 0.5°C/min, and (d) 0.1°C/min.](/cms/asset/0fd799e3-c2fa-4399-916f-d66cbb4f442e/ljfp_a_1439506_f0010_oc.jpg)
Table 1. Three experimental conditions.
Figure 4. Ice crystal growth near the ‘flashing’ at the freezing rate of 2°C/min when the temperature was: (a) −9.2°C, (b) −9.6°C, (c) −9.8°C, and (d) −10°C.
![Figure 4. Ice crystal growth near the ‘flashing’ at the freezing rate of 2°C/min when the temperature was: (a) −9.2°C, (b) −9.6°C, (c) −9.8°C, and (d) −10°C.](/cms/asset/a6e6f604-8c89-4c67-8b26-dac72a825617/ljfp_a_1439506_f0004_oc.jpg)
Figure 5. Ice crystal growth at a slower freezing rate. (a) 0.1°C/min, −16.7°C, (b) 0.1°C/min, −16.8°C, (c) 0.5°C/min, −10.5°C, and (d) 0.5°C/min, −11°C.
![Figure 5. Ice crystal growth at a slower freezing rate. (a) 0.1°C/min, −16.7°C, (b) 0.1°C/min, −16.8°C, (c) 0.5°C/min, −10.5°C, and (d) 0.5°C/min, −11°C.](/cms/asset/d0a052ed-3c71-4c11-a136-6d483782b46b/ljfp_a_1439506_f0005_oc.jpg)
Figure 6. Grey changes at the moment of ‘flashing’ during the ice crystal formation process at a freezing rate of 1.2°C/min using the image processing method (the scale of each plot refers to the range of grey values for all the pixels in each image).
![Figure 6. Grey changes at the moment of ‘flashing’ during the ice crystal formation process at a freezing rate of 1.2°C/min using the image processing method (the scale of each plot refers to the range of grey values for all the pixels in each image).](/cms/asset/e1a22fd8-ca3b-4ed8-ac1c-a9c7b07ef20b/ljfp_a_1439506_f0006_b.gif)