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Articles

Ice crystal growth of living onion epidermal cells as affected by freezing rates

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Pages 606-617 | Received 15 Jun 2017, Accepted 07 Feb 2018, Published online: 03 May 2018

Figures & data

Figure 9. The thawing performance of the different freezing rates: (a) 0.5°C/min, (b) 1.2°C/min, and (c) 2°C/min.

Figure 9. The thawing performance of the different freezing rates: (a) 0.5°C/min, (b) 1.2°C/min, and (c) 2°C/min.

Figure 10. Cell plasmolysis after thawing at the different freezing rates: (a) 2°C/min, (b) 1.2°C/min, (c) 0.5°C/min, and (d) 0.1°C/min.

Figure 10. Cell plasmolysis after thawing at the different freezing rates: (a) 2°C/min, (b) 1.2°C/min, (c) 0.5°C/min, and (d) 0.1°C/min.

Table 1. Three experimental conditions.

Figure 1. The experimental setup.

Figure 1. The experimental setup.

Figure 2. Location of the ice crystals formed at the freezing rate of 1.2°C/min.

Figure 2. Location of the ice crystals formed at the freezing rate of 1.2°C/min.

Figure 3. Ice crystal growth at different times after ‘flashing’ at the freezing rate of 1.2°C/min.

Figure 3. Ice crystal growth at different times after ‘flashing’ at the freezing rate of 1.2°C/min.

Figure 4. Ice crystal growth near the ‘flashing’ at the freezing rate of 2°C/min when the temperature was: (a) −9.2°C, (b) −9.6°C, (c) −9.8°C, and (d) −10°C.

Figure 4. Ice crystal growth near the ‘flashing’ at the freezing rate of 2°C/min when the temperature was: (a) −9.2°C, (b) −9.6°C, (c) −9.8°C, and (d) −10°C.

Figure 5. Ice crystal growth at a slower freezing rate. (a) 0.1°C/min, −16.7°C, (b) 0.1°C/min, −16.8°C, (c) 0.5°C/min, −10.5°C, and (d) 0.5°C/min, −11°C.

Figure 5. Ice crystal growth at a slower freezing rate. (a) 0.1°C/min, −16.7°C, (b) 0.1°C/min, −16.8°C, (c) 0.5°C/min, −10.5°C, and (d) 0.5°C/min, −11°C.

Figure 6. Grey changes at the moment of ‘flashing’ during the ice crystal formation process at a freezing rate of 1.2°C/min using the image processing method (the scale of each plot refers to the range of grey values for all the pixels in each image).

Figure 6. Grey changes at the moment of ‘flashing’ during the ice crystal formation process at a freezing rate of 1.2°C/min using the image processing method (the scale of each plot refers to the range of grey values for all the pixels in each image).

Figure 7. Temperature of spontaneous freezing of the onion epidermal cells at three different freezing rates.

Figure 7. Temperature of spontaneous freezing of the onion epidermal cells at three different freezing rates.

Figure 8. Changes of the cell wall gap in the frozen cells at a freezing rate of 0.1°C/min when the temperature was: (a) −16.8°C, (b) −17.3°C.

Figure 8. Changes of the cell wall gap in the frozen cells at a freezing rate of 0.1°C/min when the temperature was: (a) −16.8°C, (b) −17.3°C.