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Original Article

Comparative physicochemical stability and efficacy study of lipoid S75-biopeptides nanoliposome composite produced by conventional and direct heating methods

ORCID Icon, ORCID Icon, & ORCID Icon
Pages 1646-1660 | Received 06 Mar 2018, Accepted 20 Jul 2018, Published online: 02 Aug 2018

Figures & data

Figure 1. Changes in the physicochemical properties of the Lipoid S75-biopeptides nanoliposome composite prepared by conventional method (BLS75-CM) and Lipoid S75-biopeptides nanoliposome composite prepared by direct heating method (BLS75-DHM) during the 8 weeks of storage at 4°C. Means that do not share a common letter are significantly different at p < 0.05. (a) Changes in encapsulation efficiencies of BLS75-CM and BLS75-DHM. (b) Changes in particle size of BLS75-CM and BLS75-DHM. (c) Changes in polydispersity index (pdi) of BLS75-CM and BLS75-DHM. (d) Changes in zeta potential of BLS75-DHM and BLS75-DHM.

Figure 1. Changes in the physicochemical properties of the Lipoid S75-biopeptides nanoliposome composite prepared by conventional method (BLS75-CM) and Lipoid S75-biopeptides nanoliposome composite prepared by direct heating method (BLS75-DHM) during the 8 weeks of storage at 4°C. Means that do not share a common letter are significantly different at p < 0.05. (a) Changes in encapsulation efficiencies of BLS75-CM and BLS75-DHM. (b) Changes in particle size of BLS75-CM and BLS75-DHM. (c) Changes in polydispersity index (pdi) of BLS75-CM and BLS75-DHM. (d) Changes in zeta potential of BLS75-DHM and BLS75-DHM.

Figure 2. Cumulative release profile of the Lipoid S75-biopeptides nanoliposome composite prepared by conventional method (BLS75-CM) and Lipoid S75-biopeptides nanoliposome composite prepared by direct heating method (BLS75-DHM).

Figure 2. Cumulative release profile of the Lipoid S75-biopeptides nanoliposome composite prepared by conventional method (BLS75-CM) and Lipoid S75-biopeptides nanoliposome composite prepared by direct heating method (BLS75-DHM).

Figure 3. Transmission electron microscopy of the Lipoid S75-biopeptides nanoliposome composite prepared by conventional method (BLS75-CM) and Lipoid S75-biopeptides nanoliposome composite prepared by direct heating method (BLS75-DHM). (a) BLS75-CM nanoliposome with particle size of 141.86 nm measured by a built-in scale bar (b) BLS75-DHM nanoliposome with particle size of 182.77 nm measured by  abuilt-in scale bar.

Figure 3. Transmission electron microscopy of the Lipoid S75-biopeptides nanoliposome composite prepared by conventional method (BLS75-CM) and Lipoid S75-biopeptides nanoliposome composite prepared by direct heating method (BLS75-DHM). (a) BLS75-CM nanoliposome with particle size of 141.86 nm measured by a built-in scale bar (b) BLS75-DHM nanoliposome with particle size of 182.77 nm measured by  abuilt-in scale bar.

Table 1. Wavenumber (n, cm−1) and functional groups of FTIR spectra for EL-CM, EL–DHM, BLS75-CM and BLS75-DHM.

Figure 4. FTIR spectra of the Lipoid S75-biopeptides nanoliposome composite prepared by conventional method (BLS75-CM) and Lipoid S75-biopeptides nanoliposome composite prepared by direct heating method (BLS75-DHM) and their control capsules. (a) empty/control nanoliposome capsule prepared by conventional method (EL-CM). (b) Lipoid S75-biopeptides nanoliposome composite capsule prepared by conventional method (BLS75-CM). (c) empty/control nanoliposome prepared by direct heating methods (EL-DHM). (d) Lipoid S75-biopeptides nanoliposome composite prepared by direct heating method (BLS75-DHM).

Figure 4. FTIR spectra of the Lipoid S75-biopeptides nanoliposome composite prepared by conventional method (BLS75-CM) and Lipoid S75-biopeptides nanoliposome composite prepared by direct heating method (BLS75-DHM) and their control capsules. (a) empty/control nanoliposome capsule prepared by conventional method (EL-CM). (b) Lipoid S75-biopeptides nanoliposome composite capsule prepared by conventional method (BLS75-CM). (c) empty/control nanoliposome prepared by direct heating methods (EL-DHM). (d) Lipoid S75-biopeptides nanoliposome composite prepared by direct heating method (BLS75-DHM).

Figure 5. ACE-inhibitory activities of Lipoid S75-biopeptides nanoliposome composite prepared by conventional method (BLS75-CM), Lipoid S75-biopeptides nanoliposome composite prepared by direct heating method (BLS75-DHM) and unencapsulated (free biopeptides) before and after digestion under simulated gastrointestinal fluids (SGF and SIF). Means that do not share a common letter are significantly different (p < 0.05).

Figure 5. ACE-inhibitory activities of Lipoid S75-biopeptides nanoliposome composite prepared by conventional method (BLS75-CM), Lipoid S75-biopeptides nanoliposome composite prepared by direct heating method (BLS75-DHM) and unencapsulated (free biopeptides) before and after digestion under simulated gastrointestinal fluids (SGF and SIF). Means that do not share a common letter are significantly different (p < 0.05).