Figures & data
Figure 1. Changes in the physicochemical properties of the Lipoid S75-biopeptides nanoliposome composite prepared by conventional method (BLS75-CM) and Lipoid S75-biopeptides nanoliposome composite prepared by direct heating method (BLS75-DHM) during the 8 weeks of storage at 4°C. Means that do not share a common letter are significantly different at p < 0.05. (a) Changes in encapsulation efficiencies of BLS75-CM and BLS75-DHM. (b) Changes in particle size of BLS75-CM and BLS75-DHM. (c) Changes in polydispersity index (pdi) of BLS75-CM and BLS75-DHM. (d) Changes in zeta potential of BLS75-DHM and BLS75-DHM.
![Figure 1. Changes in the physicochemical properties of the Lipoid S75-biopeptides nanoliposome composite prepared by conventional method (BLS75-CM) and Lipoid S75-biopeptides nanoliposome composite prepared by direct heating method (BLS75-DHM) during the 8 weeks of storage at 4°C. Means that do not share a common letter are significantly different at p < 0.05. (a) Changes in encapsulation efficiencies of BLS75-CM and BLS75-DHM. (b) Changes in particle size of BLS75-CM and BLS75-DHM. (c) Changes in polydispersity index (pdi) of BLS75-CM and BLS75-DHM. (d) Changes in zeta potential of BLS75-DHM and BLS75-DHM.](/cms/asset/0dc85b0a-a9f0-4348-ba92-b355d667012b/ljfp_a_1504064_f0001_oc.jpg)
Figure 2. Cumulative release profile of the Lipoid S75-biopeptides nanoliposome composite prepared by conventional method (BLS75-CM) and Lipoid S75-biopeptides nanoliposome composite prepared by direct heating method (BLS75-DHM).
![Figure 2. Cumulative release profile of the Lipoid S75-biopeptides nanoliposome composite prepared by conventional method (BLS75-CM) and Lipoid S75-biopeptides nanoliposome composite prepared by direct heating method (BLS75-DHM).](/cms/asset/9fa32062-a2fe-4eba-a0b0-c55b7062230a/ljfp_a_1504064_f0002_oc.jpg)
Figure 3. Transmission electron microscopy of the Lipoid S75-biopeptides nanoliposome composite prepared by conventional method (BLS75-CM) and Lipoid S75-biopeptides nanoliposome composite prepared by direct heating method (BLS75-DHM). (a) BLS75-CM nanoliposome with particle size of 141.86 nm measured by a built-in scale bar (b) BLS75-DHM nanoliposome with particle size of 182.77 nm measured by abuilt-in scale bar.
![Figure 3. Transmission electron microscopy of the Lipoid S75-biopeptides nanoliposome composite prepared by conventional method (BLS75-CM) and Lipoid S75-biopeptides nanoliposome composite prepared by direct heating method (BLS75-DHM). (a) BLS75-CM nanoliposome with particle size of 141.86 nm measured by a built-in scale bar (b) BLS75-DHM nanoliposome with particle size of 182.77 nm measured by abuilt-in scale bar.](/cms/asset/969d93e0-ae9a-4fad-b4e3-5dd791e97b9e/ljfp_a_1504064_f0003_oc.jpg)
Table 1. Wavenumber (n, cm−1) and functional groups of FTIR spectra for EL-CM, EL–DHM, BLS75-CM and BLS75-DHM.
Figure 4. FTIR spectra of the Lipoid S75-biopeptides nanoliposome composite prepared by conventional method (BLS75-CM) and Lipoid S75-biopeptides nanoliposome composite prepared by direct heating method (BLS75-DHM) and their control capsules. (a) empty/control nanoliposome capsule prepared by conventional method (EL-CM). (b) Lipoid S75-biopeptides nanoliposome composite capsule prepared by conventional method (BLS75-CM). (c) empty/control nanoliposome prepared by direct heating methods (EL-DHM). (d) Lipoid S75-biopeptides nanoliposome composite prepared by direct heating method (BLS75-DHM).
![Figure 4. FTIR spectra of the Lipoid S75-biopeptides nanoliposome composite prepared by conventional method (BLS75-CM) and Lipoid S75-biopeptides nanoliposome composite prepared by direct heating method (BLS75-DHM) and their control capsules. (a) empty/control nanoliposome capsule prepared by conventional method (EL-CM). (b) Lipoid S75-biopeptides nanoliposome composite capsule prepared by conventional method (BLS75-CM). (c) empty/control nanoliposome prepared by direct heating methods (EL-DHM). (d) Lipoid S75-biopeptides nanoliposome composite prepared by direct heating method (BLS75-DHM).](/cms/asset/fe3b8cba-0ddb-4d43-a90c-f2c20c210262/ljfp_a_1504064_f0004_oc.jpg)
Figure 5. ACE-inhibitory activities of Lipoid S75-biopeptides nanoliposome composite prepared by conventional method (BLS75-CM), Lipoid S75-biopeptides nanoliposome composite prepared by direct heating method (BLS75-DHM) and unencapsulated (free biopeptides) before and after digestion under simulated gastrointestinal fluids (SGF and SIF). Means that do not share a common letter are significantly different (p < 0.05).
![Figure 5. ACE-inhibitory activities of Lipoid S75-biopeptides nanoliposome composite prepared by conventional method (BLS75-CM), Lipoid S75-biopeptides nanoliposome composite prepared by direct heating method (BLS75-DHM) and unencapsulated (free biopeptides) before and after digestion under simulated gastrointestinal fluids (SGF and SIF). Means that do not share a common letter are significantly different (p < 0.05).](/cms/asset/e57b2828-9e80-4f08-b58c-8ba1d02caae4/ljfp_a_1504064_f0005_oc.jpg)