Phytochemical screening and antioxidant properties of methanolic extract of root of Asparagus racemosus Linn

Figures & data

Table 1. Phytochemical screening of methanolic extract of Asparagus racemosus.

Compound	Test	Methanolic Plant extract
Alkaloids	Mayer’s test	+
	Hager’s test	+
	Wagner’s test	−
	Dragendroff’s test	+
Glycosides	Keller-kiliani test	+
Reducing sugar	Fehling’s test	−
Proteins	Xanthoproteic test	+
Terpenoids	Salkowski’s test	−
Phenol and Tannins	Ferric chloride test	+
Steroid	Salkowski’s test	−
Saponin	Foam test	+

+ = Detected; − = Not Detected.

Table 2. Total Phenolic and Flavonoid Content and Total Antioxidant activity of methanolic extract of A. racemosus.

Parameters	Extract
Total phenolic content (mg of GAE/g)	12.90 ± 0.002
Total flavonoid content (mg of RUE/g)	0. 80 ± 0.001
Total Antioxidant Activity (mg of AAE/g)	132.53 ± 0.12

Figure 1. DPPH radical scavenging activity of methanolic extract of A. racemosus and ascorbic acid. Values are expressed as the mean ± standard deviation (n = 3).

Figure 2. Hydroxyl radical scavenging activity of methanolic extract of A. racemosus and ascorbic acid. Values are expressed as the mean ± standard deviation (n = 3).

Figure 3. Superoxide radical scavenging activity of methanolic extract of A. racemosus and ascorbic acid. Values are expressed as the mean ± standard deviation (n = 3).

Figure 4. Hydrogen peroxide radical scavenging activity of methanolic extract of A. racemosus and ascorbic acid. Values are expressed as the mean ± standard deviation (n = 3).

Table 3. IC₅₀ values of the free radical scavenging activities of the methanolic extract of A. racemosus.

Parameters	IC50 Value (µg/mL)
	A. racemosus	Ascorbic acid
DPPH scavenging assay	9.85 ± 0.11	4.89 ± 0.38
Hydroxyl Radical scavenging assay	169.27 ± 0.31	61.78 ± 0.10
Hydrogen peroxide Radical scavenging assay	27.64 ± 0.46	55.84 ± 0.21
Super oxide radical scavenging assay	258.26 ± 0.10	248.93 ± 0.34