3,139
Views
15
CrossRef citations to date
0
Altmetric
Original Article

Structural characteristics and functional properties of walnut glutelin as hydrolyzed: effect of enzymatic modification

ORCID Icon, ORCID Icon, , &
Pages 265-279 | Received 21 Aug 2018, Accepted 22 Jan 2019, Published online: 01 Mar 2019

Figures & data

Table 1. NSI and DH of different enzymes

Figure 1. Effect of single factor experiment on NSI of modified protein. When the reaction time was 40 min and the enzyme dosage was 0.3%, the reaction temperatures were selected to be 30°C, 40°C, 50°C, 60°C, and 70°C. When the reaction temperature was 40°C and the enzyme dosage was 0.3%, the reaction time was selected to be 0, 20, 40, 60, 80 and 100 min. When the reaction temperature was 40°C and the reaction time was 40 min, the enzyme dosage was 0.1%, 0.2%, 0.3%, 0.4%, and 0.5%. Three replicates were performed for each sample

Figure 1. Effect of single factor experiment on NSI of modified protein. When the reaction time was 40 min and the enzyme dosage was 0.3%, the reaction temperatures were selected to be 30°C, 40°C, 50°C, 60°C, and 70°C. When the reaction temperature was 40°C and the enzyme dosage was 0.3%, the reaction time was selected to be 0, 20, 40, 60, 80 and 100 min. When the reaction temperature was 40°C and the reaction time was 40 min, the enzyme dosage was 0.1%, 0.2%, 0.3%, 0.4%, and 0.5%. Three replicates were performed for each sample

Figure 2. Response surfaces and contour maps of complex proteases affecting NSI of the modified protein

Figure 2. Response surfaces and contour maps of complex proteases affecting NSI of the modified protein

Figure 3. Fluorescence spectra of WG and CPMP

Figure 3. Fluorescence spectra of WG and CPMP

Table 2. Response surface test factor level for enzymatically modified treatment

Table 3. Response surface optimization test results for complex protease

Table 4. Regression model analysis of variance table

Figure 4. UV-visible spectroscopy of WG and CPMP

Figure 4. UV-visible spectroscopy of WG and CPMP

Figure 5. SEM of WG and CPMP (5000 X)

Figure 5. SEM of WG and CPMP (5000 X)

Table 5. Comparison of functional properties of WG before and after modification