Rheological properties and structure of myofibrillar protein extracted from Oratosquilla oratoria muscle as affected by ultra-high pressure

Figures & data

Figure 1. Effect of angular frequency on the viscosity of MP under different UHP conditions

Figure 2. Effect of frequency on the storage modulus G’ and tan δ of MP under different UHP conditions

Figure 3. Effect of temperature on the storage modulus G’ and tan δ of MP under different UHP conditions

Figure 4. SDS-PAGE profiles under reducing conditions of MP under different UHP conditions. M: Marker; 1: control group; 2–10: 300–5、300–6.5、300–8、350–5、350–6.5、350–8、400–5、400–6.5、400–8

Figure 5. Effect of UHP treatment on the chemical bonds of MP. Different letters on the top or bottom of bars indicate a significant difference (P < .05)

Figure 6. Circular dichroism spectrograms of MP under different UHP conditions

Table 1. Calculated structural parameters of MP samples by UHP

Groups	α-Helix	β-Sheet	β-Turn	Random coil
Control group	43.43 ± 1.52^a	24.40 ± 2.48^a	1.53 ± 1.82^e	30.60 ± 0.05^c
300–5	43.00 ± 0.70^a	6.40 ± 0.50^e	18.20 ± 0.36^a	32.40 ± 0.50^b
300–6.5	41.89 ± 0.54^ab	6.73 ± 0.27^e	18.02 ± 0.47^a	33.27 ± 0.68^ab
300–8	40.80 ± 1.02^b	6.90 ± 2.70^e	17.87 ± 0.82^a	34.43 ± 0.90^a
350–5	40.33 ± 1.13^b	12.90 ± 2.78^d	13.67 ± 2.21^bc	33.10 ± 0.94^ab
350–6.5	38.83 ± 1.46^bc	14.07 ± 2.04^d	14.27 ± 0.09^b	32.87 ± 1.05^b
350–8	37.40 ± 0.67^c	17.70 ± 1.07^c	13.30 ± 1.12^bc	30.57 ± 0.19^c
400–5	35.13 ± 0.96^d	17.00 ± 1.28^c	15.30 ± 1.64^b	32.63 ± 0.65^b
400–6.5	35.20 ± 1.13^d	22.67 ± 3.16^b	12.63 ± 1.68^c	29.47 ± 0.45^c
400–8	34.70 ± 0.91^d	24.37 ± 1.51^a	10.43 ± 1.14^d	30.53 ± 0.94^c

Different letters within the same column indicate significant between treatments (P < 0.05).