Degradation of multidrug-resistant E. coli by low pressure plasma

Figures & data

Figure 1. Bacterial disinfection process by plasma-generated reactive species

Figure 2. (a) Schematic diagram of experimental set-up. (b) Visuality of discharge at 0 mbar Pressure (c) visuality of discharge at 0.1 m bar Pressure

Figure 3. (Color online) Spectral emission profiles of ROS with and without${\mathrm{H}}_2{\mathrm{O}}_2$

Figure 4. (Color online) Normalized density of ROS with and without ${\mathrm{H}}_2{\mathrm{O}}_2$

Figure 5. (Color online) Graphical and visual representation of E.coli colonies under different experimental conditions. (a) Pure strain of E.coli cultured Agar plates with 10⁷CFU’s

While CFU’s on plasma-treated agar plates at 0.1 mbar pressure and 5.9 sccm ${\mathrm{H}}_2{\mathrm{O}}_2$spray in sterilization chamber: (b) 10 seconds plasma treatment (c) 20 seconds plasma treatment (d) 40 seconds plasma treatment.

Figure 6. (a) Inactivation efficiency of plasma on E.coli cells. (b) Kill rate for different plasma treatment times

Figure 7. (a) DNA quantification of plasma treated E.coli samples. (b) Protein leakage tests of plasma treated E.coli samples for different durations

Figure 8. Typical SEM images of plasma-treated agar plates

(a)untreated pure strain (b) 10 seconds, 0.1 mbar 5.9sccm ${\mathrm{H}}_2{\mathrm{O}}_2$ (c) 20 seconds 0.1 mbar 5.9sccm ${\mathrm{H}}_2{\mathrm{O}}_2$ (d) 0.1 mbar 5.9sccm ${\mathrm{H}}_2{\mathrm{O}}_2$ 30 seconds (e) 40 seconds 0.1 mbar 5.9sccm ${\mathrm{H}}_2{\mathrm{O}}_2$