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Original Article

In-vitro antioxidant and in-vivo anti-aging with stress resistance on Caenorhabditis elegans of herbaceous peony stamen tea

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Pages 1349-1366 | Received 14 May 2021, Accepted 07 Aug 2021, Published online: 20 Aug 2021

Figures & data

Figure 1. Isolation of P. lactiflora stamens. A: Blooming flower; B: Remaining part of flower after removing the petals; C: Separate stamens; D: Stamens after a series of treatments

Figure 1. Isolation of P. lactiflora stamens. A: Blooming flower; B: Remaining part of flower after removing the petals; C: Separate stamens; D: Stamens after a series of treatments

Figure 2. Reducing capacity and free radical scavenging activity of P. lactiflora stamen extract. The values represented the mean ± SD, and different letters indicate significant differences according to Duncan’s multiple range test (P < .05)

Figure 2. Reducing capacity and free radical scavenging activity of P. lactiflora stamen extract. The values represented the mean ± SD, and different letters indicate significant differences according to Duncan’s multiple range test (P < .05)

Figure 3. Effects of P. lactiflora stamen extract on safety of C. elegans. A: Effects of stamen extract on lethality, growth, locomotion behavior and reproduction of nematodes; B: Effects of stamen extract on intestinal autofluorescence of nematodes. The values represented the mean ± SD, and different letters indicate significant differences according to Duncan’s multiple range test (P < .05)

Figure 3. Effects of P. lactiflora stamen extract on safety of C. elegans. A: Effects of stamen extract on lethality, growth, locomotion behavior and reproduction of nematodes; B: Effects of stamen extract on intestinal autofluorescence of nematodes. The values represented the mean ± SD, and different letters indicate significant differences according to Duncan’s multiple range test (P < .05)

Figure 4. Effects of P. lactiflora stamen extract on lifespan of C. elegans. A: Lifespan curves of nematodes treated with stamen extract; B: Comparison of mean lifespan and maximal lifespan in nematodes treated with stamen extract. The values represented the mean ± SD, and different letters indicate significant differences according to Duncan’s multiple range test (P < .05)

Figure 4. Effects of P. lactiflora stamen extract on lifespan of C. elegans. A: Lifespan curves of nematodes treated with stamen extract; B: Comparison of mean lifespan and maximal lifespan in nematodes treated with stamen extract. The values represented the mean ± SD, and different letters indicate significant differences according to Duncan’s multiple range test (P < .05)

Figure 5. Effects of P. lactiflora stamen extract on locomotion behavior of C. elegans. The values represented the mean ± SD, and different letters indicate significant differences according to Duncan’s multiple range test (P < .05)

Figure 5. Effects of P. lactiflora stamen extract on locomotion behavior of C. elegans. The values represented the mean ± SD, and different letters indicate significant differences according to Duncan’s multiple range test (P < .05)

Figure 6. Effects of P. lactiflora stamen extract on intestinal autofluorescence and reproduction of C. elegans. A: Effects of stamen extract on intestinal autofluorescence of nematodes; B: Fluorescence intensity of intestinal autofluorescence; C Effects of stamen extract on reproduction of nematodes. The values represented the mean ± SD, and different letters indicate significant differences according to Duncan’s multiple range test (P < .05)

Figure 6. Effects of P. lactiflora stamen extract on intestinal autofluorescence and reproduction of C. elegans. A: Effects of stamen extract on intestinal autofluorescence of nematodes; B: Fluorescence intensity of intestinal autofluorescence; C Effects of stamen extract on reproduction of nematodes. The values represented the mean ± SD, and different letters indicate significant differences according to Duncan’s multiple range test (P < .05)

Figure 7. Effects of P. lactiflora stamen extract on stress resistance of C. elegans. A: Lifespan curves of C. elegans treated with stamen extract and their comparison of mean lifespan and maximal lifespan under heat stress; B: Lifespan curves of C. elegans treated with stamen extract and their comparison of mean lifespan and maximal lifespan under oxidative stress. The values represented the mean ± SD, and different letters indicate significant differences according to Duncan’s multiple range test (P < .05)

Figure 7. Effects of P. lactiflora stamen extract on stress resistance of C. elegans. A: Lifespan curves of C. elegans treated with stamen extract and their comparison of mean lifespan and maximal lifespan under heat stress; B: Lifespan curves of C. elegans treated with stamen extract and their comparison of mean lifespan and maximal lifespan under oxidative stress. The values represented the mean ± SD, and different letters indicate significant differences according to Duncan’s multiple range test (P < .05)
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