Antioxidant, Anti-Melanogenic and Anti-Wrinkle Effects of Phellinus vaninii

Figures & data

Table 1. Total polyphenol and flavonoid contents of methanol and hot water extract from fruiting bodies of Phellinus vaninii.

Samples	Yields (% w/w)	Phenolics content (mg GAE/g dry extract wt)	Flavonoids content (mg QE/g dry extract wt)
Methanol extract	10.91	31.67 ± 1.25	6.83 ± 0.85
Hot water extract	6.34	16.33 ± 062	4.50 ± 0.41

GAE: gallic acid equivalents; QE: quercetin equivalent; wt: weight.

Values are means ± SD (n = 3).

Figure 1. Cytotoxicity effects of methanol and hot water extracts from fruiting bodies of Phellinus vaninii against B16-F10 melanoma cells. Values are means ± SD (n = 3).

Figure 2. Antioxidant activities of methanol and hot water extracts from fruiting bodies of Phellinus vaninii. (A) DPPH radical scavenging activity; (B) Lipid peroxidation inhibitory activity; (C) Hydroxyl radical (OH⁻) scavenging activity. Values are means ± SD (n = 3). BHT, butylated hydroxytoluene; DPPH, 1,1-Diphenyl-2-picryl-hydrazy. ***p < .001, **p < .01, *p < .05 vs. BHT group; ^##p < .01, ^#p < .05 vs. Methanol extract group.

Figure 3. Xanthine oxidase inhibitory activity of methanol and hot water extracts from fruiting body of Phellinus vaninii. Methanol extract was extracted with 80% methanol; Hot water extract was extracted with hot water. Values are means ± standard deviation (SD, n = 3). ***p < .001, **p < .01, *p < .001 vs. allopurinol group; ^###p < .001, ^##p < .01, ^#p < .05 vs. Methanol extract group.

Figure 4. Tyrosinase and DOPA auto-oxidation inhibitory activities of methanol and hot water extracts from fruiting bodies of Phellinus vaninii. (A) Tyrosinase inhibitory activity; (B) DOPA auto-oxidation inhibitory activity. Values are means ± standard deviation (SD, n = 4). ***p < .001, *p < .05 vs. Kojic acid group; ^###p < .001, ^#p < .05 vs. Methanol extract group.

Figure 5. Cellular tyrosinase and melanin synthesis activities of methanol and hot water extracts from fruiting bodies of Phellinus vaninii. (A) Tyrosinase activity in B16-F10 melanoma cells; (B) Melanin synthesis activity in B16-F10 melanoma cells. Values are means ± standard deviation (SD, n = 4). ***p < .001, **p < .01, *p < .05 vs. Arbutin group; ^###p < .001, ^##p < .01 vs. Methanol extract group.

Figure 6. Elastase and collagenase inhibitory activities of methanol and hot water extracts from fruiting bodies of Phellinus vaninii. Values are means ± standard deviation (SD, n = 4). (A) Collagenase inhibitory activity; (B) Elastase inhibitory activity. EGCG, epigallocathechin-3-gallate. ***p < .001 vs. EGCG group; ^###p < .001, ^##p < .01, ^#p < .05 vs. Methanol extract group.