Molecular Identification of a New Species of Absidia (Cunninghamellaceae, Mucorales) Isolated from Soil in Korea

Figures & data

Table 1. GenBank accession numbers of sequences used for the phylogenetic analyses in this study.

Species name	Strain number	GenBank accession numbers
		ITS	LSU
Absidia microsporangia sp. nov.	KNUF-22-121A	OQ868364	OQ868366
Absidia microsporangia sp. nov.	KNUF-22-126A	OQ868365	OQ868367
Absidia microsporangia sp. nov.	KNUF-22-316^T	PP264375	PP264469
Absidia ampullaceal	CGMCC 3.16054^T	MZ354138	MZ350132
Absidia brunnea	CGMCC 3.16055^T	MZ354139	MZ350133
Absidia chinensis	CGMCC 3.16057	MZ354141	MZ350135
Absidia digitula	CGMCC 3.16058	MZ354142	MZ350136
Absidia ovalispora	CGMCC 3.16018	MW264071	MW264130
Absidia ovalispora	CGMCC 3.16019^T	NR_176748	MW264131
Absidia radiata	CGMCC 3.16257^T	ON074698	ON074684
Absidia radiata	XY09330-1	ON074699	ON074685
Absidia saloaensis	URM 8209	MN953781	MN953783
Absidia soli	MFLUCC 20-0089^T	NR_172306	NG_075368
Absidia soli	MFLU-20-0414	MT396373	MT393988
Absidia yunnanensis	CGMCC 3.16259^T	ON074700	ON074687
Absidia yunnanensis	XY09528	ON074701	ON074686
Cunninghamella elegans	CBS 167.53	JN205882	HM849700

ITS: internal transcribed spacer regions; LSU: partial sequence of large subunit 28S rDNA.

The newly generated sequences are indicated in bold.

Table 2. Morphological comparison between Absidia microsporangia sp. nov. (KNUF-22-316A^T) and the phylogenetically closest species of Absidia.

Strain name	A. microsporangia sp. nov. KNUF-22-316^T^a	A. yunnanensis CGMCC 3.16259^T^b	A. radiata CGMCC 3.16257^T^b	A. ampullacea CGMCC 3.16054^T^c
Colonies on MEA	59 mm for 7 d	65 mm for 7 d	65 mm for 7 d	Reaching 90 mm in 10 d
Sporangiophores (µm)	36.1–102.8 × 1.8–3.1	29.0–159.0 × 2.0–5.0	45.0–273.0 × 3.0–5.0	30.0–320.0 × 2.5–5.5
Sporangia (µm)	Oval to subglobose 9.2–16.6 × 8.4–16.6	Subglobose or pyriform 16.0–27.5 × 16.5–27.0	Subglobose or pyriform 17.5–33.5 × 18.5–30.0	Oval to pyriform 17.5–37.5 × 17.5–45.0
Collars	Present	Absent or present	Absent	Absent
Columellae (µm)	Oval to subglobose, hyaline 6.2–11.8 × 5.6–11.7	Oval, hyaline, or light green 6.5–18.5 × 7.5–22.0	Oval, hyaline 13.5–22.5 × 14.0–24.0	Hemispherical, hyaline 8.5–22.5 × 10.5–24.0
Projection	Hyaline to light brown 1.2–2.6 µm in length	Subhyaline 1.5–3.5 µm in length	Subhyaline 2.0–4.5 µm in length	Hyaline 0.5–2.0 µm in length
Sporangiospores (µm)	Oval or subglobose, hyaline 2.9–5.8 × 2.0–4.4	Cylindrical, light green 3.5–5.0 × 2.0–4.0	Oval, subhyaline 3.0–5.0 × 2.0–3.5	Oval or subglobose, hyaline 3.0–4.5 × 2.5–4.0

^a Fungal strain studied in this research.

^b Source of descriptions [10].

^c Source of description [2].

Figure 1. Morphology of Absidia microsporangia sp. nov. KNU-22-316^T. (A, B) Colonies on PDA and MEA at 25 °C for seven days, obverse and reverse, respectively; (C) swelling on sporangiophores; (D, E) sporangium; (F) columellae, collars, and projections; (G) rhizoid; (H) sporangiophores. Scale bars: C–I = 10 μm. Black arrows, collars; a red arrow, projection.

Figure 2. Neighbor-joining phylogenetic tree based on ITS regions and LSU gene sequences showing the phylogenetic position of the three isolated strains among the related strains in the genus Absidia. Cunninghamella elegans CBS 167.53 was used as the outgroup. The strains isolated in this study are indicated in bold, and the numbers above the branches represent the bootstrap values (>70%) obtained for 1000 replicates. Scale bar = 0.02 substitutions per nucleotide position.

Zhao H, Nie Y, Zong TK, et al. Species diversity and ecological habitat of Absidia (Cunninghamellaceae, Mucorales) with emphasis on five new species from forest and grassland soil in China. J Fungi. 2022;8:1–19.

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