Molecular cloning and expression analysis of seven sucrose synthase genes in bamboo (Bambusa emeiensis): investigation of possible roles in the regulation of cellulose biosynthesis and response to hormones

Figures & data

Table 1. Physical and chemical properties of the protein encoded by SUS genes from Bambusa emeiensis, Bambusa oldhamii and Oryza sativa.

Gene name	Amino acid	Molecular mass (kDa)	Acidic amino acid	Alkaline amino acid	pI	Grand average of hydropathicity
BeSUS1	816	92.85	103	85	5.97	−0.450
BeSUS2	612	69.29	76	58	5.62	−0.250
BeSUS3	816	92.78	101	86	6.06	−0.261
BeSUS4	808	91.97	103	90	6.06	−0.578
BeSUS5	816	92.75	102	86	6.01	−0.261
BeSUS6	841	95.57	105	94	6.34	−0.26
BeSUS7	849	96.69	109	111	7.78	−0.414
BoSUS1	816	92.88	102	85	6.03	−0.261
BoSUS2	808	92.25	104	90	6.03	−0.578
BoSUS3	816	92.98	102	87	6.10	−0.261
BoSUS4	808	92.12	105	91	5.98	−0.595
OsSUS1	816	92.91	103	86	5.94	−0.261
OsSUS2	808	92.08	102	88	6.01	−0.517

Figure 1. Phylogenetic analysis of B. emeiensis SUS genes and other plant SUS homologs.

Fugure 2. qRT-PCR analysis of BeSUS genes in different B. emeiensis tissues, including root (R), stem (S), unexpanded leaves (UL), mature leaves (ML) and shoot (30 and 100 cm).

Figure 3. BeSUSs expression response to ABA stress in leaves.

Figure 4. BeSUSs expression response to MeJA stress in leaves.

Figure 5. Effects of 2,6-DCB on cellulose synthesis in B. emeiensis. Cellulose content (a) in mock-treated plants (0.04% dimethyl sulfoxide and 70% alcohol) and plants treated with 1 mmol/L 2,6-DCB. FTIR spectrum (b) obtained from mock-treated and 2,6-DCB-treated plants.

Figure 6. Relative transcript levels of BeSUS1–7 in response to 2,6-DCB.