cDNA-AFLP analysis of salicylic acid- and calcium chloride-induced transcript derived fragments under drought in tomato (Solanum lycopersicum)

Figures & data

Figure 1. Plant phenotype on the third day of drought stress. CK1, control group with normal watering; CK2, control group with drought only; S0.3, exogenous SA group with drought; and C10, exogenous CaCl₂ group with drought.

Figure 2. Seven differential expression patterns were used to analyze the drought resistance response induced by exogenous SA and CaCl₂ in tomato. (A) Expressed in all groups; (B) induced by combination of drought and CaCl₂ (expressed in C10 only); (C) induced by combination of drought and SA (expressed in S0.3 only); (D) repressed by drought stress (expressed in CK1, CKC and CKS); (E) repressed by combination of drought and exogenous substances (expressed in CK1, CK2, CKC and CKS); (F) repressed by drought or exogenous substances (expressed in CK1 only); and (G) induced by drought stress (expressed in CK2, C10 and S0.3).

Table 1. Homology analysis of SA- and Ca²⁺-induced differentially expressed transcripts under drought in tomato.

TDF	Accession number	Homology to sequences in the NCBI database (Accession)	E-value	Expression pattern	Identity (%)
TDF3	JZ948559	Von Willebrand factor A domain-containing protein, transcript variant X3 (XM_010320173.1)	3e − 73	E	95
TDF7	JZ948562	B2 protein (LOC101243655), transcript variant X4 (XM_004231734.2)	1e − 82	E	97
TDF10	JZ948565	PREDICTED: titin (LOC101259599), transcript variant X10 (XM_010318008.1)	2e − 51	C	99
TDF11	JZ948566	PREDICTED: 4-hydroxyphenylpyruvate dioxygenase-like (LOC101257377) (XM_004240123.2)	2e − 126	G	99
TDF15	JZ948569	plasma membrane H+-ATPase (LHA2) (NM_001247548.1)	9e − 119	F	96
TDF16	JZ948570	PREDICTED: probable transcription factor PosF21 (LOC101250636) (XM_004230853.2)	5e − 116	C	97
TDF20	JZ948572	PREDICTED: Solanum lycopersicum, nucleolin (LOC101258799), transcript variant X5, mRNA (XM_004248939.2)	0.0	E	99
TDF22	JZ948576	PREDICTED: Solanum lycopersicum titin (LOC101259599), transcript variant X10, mRNA (XM_010318008.1)	2e − 114	C	98
TDF24	JZ948576	voucher H7072 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial (EU827430.1)	8e − 12	B	85
TDF29	JZ948581	ARGOS (LOC100316893) (NM_001247750.2)	7e − 17	G	95
TDF34	JZ948586	Solanum lycopersicum transmembrane amino acid transporter family protein (LOC101261384), mRNA	1e − 04	B	100
TDF44	JZ948594	PREDICTED: microsomal glutathione S-transferase 3 (LOC101263072) (XM_004232613.2)	5e − 37	F	82
TDF50	JZ948600	PREDICTED: lisH domain and HEAT repeat-containing protein KIAA1468 homolog (LOC101253751) (XM_004233660.2)	2e − 41	C	96
TDF53	JZ948603	PREDICTED: calcium-dependent protein kinase 11 (LOC101251099), transcript variant X2 (XM_010324456.1)	3e − 26	D	89
TDF54	JZ948604	PREDICTED: chitin elicitor receptor kinase 1-like (LOC102595953), transcript variant X2 (XM_006361830.2)	0.14	D	77
TDF55	JZ948605	PREDICTED: calcium-dependent protein kinase 11 (XP_015077134.1)	0.013	D	71
TDF57	JZ948607	PREDICTED: UDP-D-apiose/UDP-D-xylose synthase 2 (LOC101247451) (XM_004228410.2)	3e − 102	F	99
TDF59	JZ948609	PREDICTED: elongation factor G, chloroplastic (LOC101245683) (XM_004245684.2)	4e − 87	E	96
TDF62	JZ948612	phytochromobilin synthase (AUREA) (NM_001247204.3)	3e − 88	C	98
TDF63	JZ948613	PREDICTED: Solanum lycopersicum transcription factor WER-like (LOC101245233), mRNA (XM_004235149.2)	2e − 20	F	76
TDF64	JZ948614	PREDICTED: heat shock protein 83-like (LOC101260143) (XM_004240669.2)	2e − 39	C	89
TDF65	JZ948615	PREDICTED: proteinase inhibitor 1-like (LOC101266562) (XM_004247647.2)	4e − 147	F	99
TDF67	JZ948617	PREDICTED: coatomer subunit beta'-3-like (LOC107007027), transcript variant X4 (XR_001455002.1)	1e − 06	B	100
TDF73	JZ948620	PREDICTED: Ciona intestinalis mitogen-activated protein kinase kinase kinase 4 (LOC778975), mRNA (XM_009863224.1)	0.24	F	91
TDF74	JZ948621	PREDICTED: ABC transporter G family member 11-like (LOC102600699) (XM_006351457.2)	0.083	B	70
TDF75	JZ948622	PREDICTED: Solanum pennellii elongation factor G, chloroplastic (LOC107028360), mRNA (XM_015229387.1)	2E − 70	G	93
TDF78	JZ948625	PREDICTED: protein ECERIFERUM 26-like (LOC101245886) (XM_004247830.2)	2e − 74	E	99
TDF81	JZ948628	PREDICTED: proteinase inhibitor 1-like (LOC101266562) (XM_004247647.2)	7e − 109	D	98
TDF82	JZ948629	PREDICTED: elongation factor G, chloroplastic (LOC107028360) (XM_015229387.1)	2e − 59	D	97
TDF87	JZ948634	PREDICTED: probable trans-2-enoyl-CoA reductase, mitochondrial (LOC101247598) (XM_004252983.2)	1e − 95	F	99
TDF93	JZ948638	PREDICTED: protein transport protein SEC13 homolog B (LOC107011415) (XM_015210912.1)	1e − 14	F	89
TDF98	JZ948641	PREDICTED: RINT1-like protein MAG2 (LOC101250005) (XM_004236156.2)	4e − 111	B	99
TDF99	JZ948642	PREDICTED: abscisic acid 8′-hydroxylase 1 (LOC101249565) (XM_004244388.2)	1e − 20	F	89
TDF100	JZ948643	PREDICTED: LRR receptor-like serine/threonine-protein kinase FLS2 (LOC101259509) (XM_004233819.2)	6e − 87	C	92

Note: Expression pattern B represents genes induced by combination of drought and CaCl₂; Expression pattern C represents genes induced by combination of drought and SA; Expression pattern D represents genes repressed by drought stress; Expression pattern E represents genes repressed by combination of drought and exogenous substances; Expression pattern F represents genes repressed by drought or exogenous substances; Expression pattern G represents genes induced by drought stress.

Figure 3. qRT-PCR analysis of 12 differentially expressed transcripts induced by exogenous SA and CaCl₂ under drought stress in tomato. Note: Mean values (n = 3) with standard deviation (±SD).

Figure 4. Functional annotation distribution of 34 differentially expressed fragments.