Identification of candidate genes associated with photosynthesis in eggplant under elevated CO₂

Figures & data

Table 1. Primers used for qRT-PCR analysis.

Gene ID	Primer sequence
	Forward primer (5′–3′)	Reverse primer (5′–3′)
Sme2.5_00502.1_g00014	GTTGAGCTTTTGCAGCGTGA	TGGAGAACAACCGAGCAACA
Sme2.5_13003.1_g00001	GGTGGTGATCGAGATGGCAA	TTTGCTACAGAACCAGGGGC
Sme2.5_03383.1_g00002	GACGTTGTCAGGGCAAACAC	CGACCTCAGTCCCAGCATTT
Sme2.5_04464.1_g00001	CAGAGTTGGCACACGAGGAA	GCCAAGTCAGGATACGAGGC
Sme2.5_00037.1_g00011	CGAGCACCCTTGTCCTTCTT	TGGAGAGGGCAAGTACCAGA
Sme2.5_00497.1_g00006	GGTCCTTGTGGGGAGCTAAG	GCCCCAGTCATCCTTTCCAG
Sme2.5_01812.1_g00005	CAATGCGGTTCACACGAGC	GGTGTTCCAGGCTCTTATCCA
Sme2.5_00003.1_g00016	CGGTGGGCACTGATCGTAAA	GATTCGTTGCGAGCGAGGTA
Sme2.5_00457.1_g00010	ATGAATGCCTCGGTGCTGTT	AGCAGCACTGGTTTACGGAT
Sme2.5_02344.1_g00010	CTGTTGCGAGTAGTGGAGGG	CAGAGCCACAGCAACCTGAT
Sme2.5_01232.1_g00025	TGGAAAATCTCTCAGCGCCA	AAGATCCGCCATTAGGCCAC
Sme2.5_00372.1_g00006	CACCAACAACGAACTCTGCG	AAGGCAGGTGGTGAGTTAGC

Figure 1. Effect of elevated CO₂ on eggplant growth. Dynamics of plant height (A), stem diameter (B), plant width (C) and leaf numbers (D) of eggplant.

Note: Bars represent mean values ± SD (n = 3) of triplicate repeats. Upper case letters in each panel indicate significant differences among samples by Student’s t-test at p < 0.01 and lower case letters indicate significant differences at p < 0.05.

Table 2. Effect of elevated CO₂ on photosynthesis in eggplant.

Treatme-nt	Pnmax (μmol CO2 m^−2 s^−1)	Lsp (Lux)	Lcp (Lux)	Rday (μmol·m^−2·s^−1)	AQE	Gs (mmol·m^−2·s^−1)	Ci (μmol·mol^−1)
Control	24.34 ± 1.5^Bb	1414.4 ± 2.80 ^Aa	32.0 ± 3.00 ^Aa	3.15 ± 0.05^Aa	0.050 ± 0.005^Bb	0.73 ± 0.14^Aa	314.33 ± 0.07^Bb
Elevated CO2	45.30 ± 0.9^Aa	2356.8 ± 3.50 ^Aa	28.8 ± 1.00 ^Bb	2.29 ± 0.39^Aa	0.079 ± 0.004^Aa	0.54 ± 0.04^Bb	635.13 ± 0.05^Aa

Note: AQE: apparent quantum efficiency; Ci: intercellular CO₂ concentration; Gs: stomatal conductance; Lcp: light compensation point; Lsp: light saturation point; Pnmax: maximum net photosynthetic rate; Rday: dark respiration rate.

Upper case letters in each panel indicate significant differences among samples by Student’s t-test at p < 0.01 and lower case letters indicate significant differences at p < 0.05.

Figure 2. Gene ontology(GO) analysis of differential expressed genes (DEGs) from eggplant. A total of 169 DEGs were categorized into 53 function items.

Figure 3. KEGG analysis of differential expressed genes(DEGs). A total of 49 pathways were identified.

Figure 4. qRT-PCR validation of 12 differential expressed genes (DEGs). The FPKM value of each gene from RNA-seq was marked in panel under the diagram of qRT-PCR.

Data availability

The authors confirm that the data supporting the findings of this study are available within the article and its supplementary materials.