Figures & data
Table 1. Experimental variables at two levels used for the antifungal effect of the cell-free filtrates from HU2014 culture using PB design.
Table 2. PB design for the optimisation of parameters influencing antifungal activity of the cell-free filtrates from HU2014 culture.
Table 3. Input factors with their ranges and levels.
Table 4. RSM-based FCCD experimental design matrix for three factors.
Figure 1. Scanning broad spectrum of the cell-free filtrates of HU2014 culture broth by spectrophotometer. IA1-10 represent the Abs lines of different sampling times.
![Figure 1. Scanning broad spectrum of the cell-free filtrates of HU2014 culture broth by spectrophotometer. IA1-10 represent the Abs lines of different sampling times.](/cms/asset/7bb65ed3-27fc-43b3-b8e3-6758fa764579/tbeq_a_2178822_f0001_c.jpg)
Figure 2. Scanning broad spectrum of four fractions extracted from the cell-free filtrates of HU2014 culture broth by spectrophotometry (A) and the antifungal activity of four fractions against R. solani YL-3 on day 3 (B). Values are means ± SD of three independent experiments. Means with the same letter for inhibition rate are not significantly different (p<0.05). F2, F4, F6 and F8 represent one of fractions, respectively.
![Figure 2. Scanning broad spectrum of four fractions extracted from the cell-free filtrates of HU2014 culture broth by spectrophotometry (A) and the antifungal activity of four fractions against R. solani YL-3 on day 3 (B). Values are means ± SD of three independent experiments. Means with the same letter for inhibition rate are not significantly different (p<0.05). F2, F4, F6 and F8 represent one of fractions, respectively.](/cms/asset/5d6b5148-283c-4b74-9e2d-2b4ab598f70c/tbeq_a_2178822_f0002_c.jpg)
Figure 3. Antifungal activities of cell-free filtrates of HU2014 culture broth against R. solani YL-3 and biomass growth of HU2014 in different conditions: Different basal media (A) and different culture conditions (B-F). Each treatment was repeated three times with three biological replicates, and the data show Means ± SD. G1 represents Gause’s No.1 medium. Cza represents Czapek’s medium.
![Figure 3. Antifungal activities of cell-free filtrates of HU2014 culture broth against R. solani YL-3 and biomass growth of HU2014 in different conditions: Different basal media (A) and different culture conditions (B-F). Each treatment was repeated three times with three biological replicates, and the data show Means ± SD. G1 represents Gause’s No.1 medium. Cza represents Czapek’s medium.](/cms/asset/ebfe414a-db42-4a64-8ff9-b0955360eb64/tbeq_a_2178822_f0003_b.jpg)
Figure 4. The antifungal activities of cell-free filtrates of HU2014 culture broth with different medium components against R. solani YL-3 and the biomass of the strain (ACE), and variation of the R. solani YL-3 (BDF) inhibition rate with different concentration of medium components.
![Figure 4. The antifungal activities of cell-free filtrates of HU2014 culture broth with different medium components against R. solani YL-3 and the biomass of the strain (ACE), and variation of the R. solani YL-3 (BDF) inhibition rate with different concentration of medium components.](/cms/asset/76ba6f09-8ff8-431b-b00c-edb0d688178f/tbeq_a_2178822_f0004_b.jpg)
Figure 5. Pareto chart of the standardised effects for eight medium factors on the antifungal activities of the cell-free filtrates of HU2014 culture broth.
![Figure 5. Pareto chart of the standardised effects for eight medium factors on the antifungal activities of the cell-free filtrates of HU2014 culture broth.](/cms/asset/52f31baf-b6fc-40a3-a859-6de7ae81b798/tbeq_a_2178822_f0005_b.jpg)
Table 5. ANOVA for the experimental result of PB design.
Table 6. ANOVA for Quadratic RSM model details.
Supplemental Material
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The data supporting the findings of this study are available from the authors upon reasonable request.