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Journal of Enzyme Inhibition and Medicinal Chemistry Volume 35, 2020 - Issue 1
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Original Article

Use of an immobilised thermostable α-CA (SspCA) for enhancing the metabolic efficiency of the freshwater green microalga Chlorella sorokiniana

Giovanna Salbitania Department of Biology, University of Naples Federico II, Napoli, ItalyView further author information
,
Sonia Del Preteb Department of Biology, Agriculture and Food Sciences, CNR, Institute of Biosciences and Bioresources, Napoli, ItalyView further author information
,
Francesco Bolinesia Department of Biology, University of Naples Federico II, Napoli, ItalyView further author information
,
Olga Mangonia Department of Biology, University of Naples Federico II, Napoli, ItalyORCID Iconhttps://orcid.org/0000-0001-7789-0820View further author information
ORCID Icon,
Viviana De Lucab Department of Biology, Agriculture and Food Sciences, CNR, Institute of Biosciences and Bioresources, Napoli, ItalyView further author information
,
Vincenzo Carginaleb Department of Biology, Agriculture and Food Sciences, CNR, Institute of Biosciences and Bioresources, Napoli, ItalyView further author information
,
William A. Donaldc School of Chemistry, University of New South Wales, Sydney, AustraliaView further author information
,
Claudiu T. Supuranc School of Chemistry, University of New South Wales, Sydney, Australia;d Department of NEUROFARB, Section of Pharmaceutical and Nutraceutical Sciences, University of Florence, Polo Scientifico, Firenze, ItalyORCID Iconhttps://orcid.org/0000-0003-4262-0323View further author information
ORCID Icon,
Simona Carfagnaa Department of Biology, University of Naples Federico II, Napoli, ItalyCorrespondence[email protected]
ORCID Iconhttps://orcid.org/0000-0002-9862-1280View further author information
ORCID Icon &
Clemente Capassob Department of Biology, Agriculture and Food Sciences, CNR, Institute of Biosciences and Bioresources, Napoli, ItalyCorrespondence[email protected]
ORCID Iconhttps://orcid.org/0000-0003-3314-2411View further author information
ORCID Icon show all
Pages 913-920 | Received 25 Feb 2020, Accepted 16 Mar 2020, Published online: 30 Mar 2020

Figures & data

Figure 1. Microalgal endogenous activity revealed by the protonography analysis. Legend: Lane 1, molecular markers; Lane 2, C. sorokiniana cellular extract; Lane 3, commercial bovine CA used as positive controls. The arrows identified the yellow bands corresponding to CO2 hydratase activity due to the microalgal CAs.

Figure 1. Microalgal endogenous activity revealed by the protonography analysis. Legend: Lane 1, molecular markers; Lane 2, C. sorokiniana cellular extract; Lane 3, commercial bovine CA used as positive controls. The arrows identified the yellow bands corresponding to CO2 hydratase activity due to the microalgal CAs.

Figure 2. Cellular density (cells mL−1) in control and experimental cultures (Bic, M-SspCA-0, M-SspCA-2) of Chlorella sorokiniana. Error bars represent SD (n = 3).

Figure 2. Cellular density (cells mL−1) in control and experimental cultures (Bic, M-SspCA-0, M-SspCA-2) of Chlorella sorokiniana. Error bars represent SD (n = 3).

Figure 3. Average cell diameter of C. sorokiniana cells in control and experimental cultures (Bic, M-SspCA-0, M-SspCA-2). Error bars represent SD (n = 3).

Figure 3. Average cell diameter of C. sorokiniana cells in control and experimental cultures (Bic, M-SspCA-0, M-SspCA-2). Error bars represent SD (n = 3).

Figure 4. Maximum quantum yield (Fv/Fm) in Chlorella sorokiniana control and experimental cultures (Bic, M-SspCA-0, M-SspCA-2). Error bars represent SD (n = 3).

Figure 4. Maximum quantum yield (Fv/Fm) in Chlorella sorokiniana control and experimental cultures (Bic, M-SspCA-0, M-SspCA-2). Error bars represent SD (n = 3).

Figure 5. Total chlorophyll content in control C. sorokiniana cells and experimental cultures (Bic, M-SspCA-0, M-SspCA-2). Error bars represent SD (n = 3).

Figure 5. Total chlorophyll content in control C. sorokiniana cells and experimental cultures (Bic, M-SspCA-0, M-SspCA-2). Error bars represent SD (n = 3).

Figure 6. Total carotenoids content in control C. sorokiniana cells and experimental cultures (Bic, M-SspCA-0, M-SspCA-2). Error bars represent SD (n = 3).

Figure 6. Total carotenoids content in control C. sorokiniana cells and experimental cultures (Bic, M-SspCA-0, M-SspCA-2). Error bars represent SD (n = 3).

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