Antioxidant activity and characterization of flavonoids and phenolic acids of Ammoides atlantica by RP–UHPLC–ESI–QTOF–MSⁿ

Abstract

In this study, phytochemical profiling of hydro-alcoholic extract of Ammoides atlantica aerial parts has been carried out using RP-UHPLC-ESI-QTOF-MS in negative ionization mode Chemical characterization was established according to the MS and MS/MS spectra. A total of 66 chemical compounds were detected. Among these, 45 compounds were identified: hydroxycinnamic acid and derivatives (26), hydroxybenzoic acids (4), flavones (11), flavonols (3), and a lignan. Total phenolics (371.57 mg/g) and total flavonoids (41.02 mg/g) contents were also determined. Moreover, the antioxidant activity of A. atlantica extract was also studied by six methods: DPPH (IC₅₀: 23.31 µg/mL), ABTS⁺ (IC₅₀: 11.31 µg/mL), O₂^- DMSO alkaline (IC₅₀: 3.19 µg/mL), ferrous ions chelating assays (IC₅₀: 102.35 µg/mL), reducing power (A_0.50: 92.70 µg/mL) and CUPRAC (A_0.50: 13.56 µg/mL) assays. These results suggest that the antioxidant activity of the hydroalcoholic extract was comparable to common antioxidant additives in most of the tests, representing a good alternative.

Graphical Abstract

Keywords:

• Ammoides atlantica
• antioxidant activity
• flavonoids
• phenolic acids
• RP–UHPLC–ESI–QTOF–MS2

Additional information

Funding

The authors are grateful to the MESRS-DGRSDT (Algeria) for financial support.