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Review

Insights and prospects for ion mobility-mass spectrometry in clinical chemistry

ORCID Icon, ORCID Icon & ORCID Icon
Pages 17-31 | Received 02 Sep 2021, Accepted 23 Dec 2021, Published online: 17 Jan 2022
 

ABSTRACT

Introduction

Ion mobility-mass spectrometry is an emerging technology in the clinical setting for high throughput and high confidence molecular characterization from complex biological samples. Ion mobility spectrometry can provide isomer separations on the basis of molecular structure, the ability of which is increasing through technological developments that afford enhanced resolving power. Integrating multiple separation dimensions, such as liquid chromatography-ion mobility-mass spectrometry (LC-IM-MS) provide dramatic enhancements in the mitigation of molecular interferences for high accuracy clinical measurements.

Areas covered

Multidimensional separations with LC-IM-MS provide better selectivity and sensitivity in molecular analysis. Mass spectrometry imaging of tissues to inform spatial molecular distribution is improved by complementary ion mobility analyses. Biomarker identification in surgical environments is enhanced by intraoperative biochemical analysis with mass spectrometry and holds promise for integration with ion mobility spectrometry. New prospects in high resolving power ion mobility are enhancing analysis capabilities, such as distinguishing isomeric compounds.

Expert Opinion

Ion mobility-mass spectrometry holds many prospects for the field of isomer identification, molecular imaging, and intraoperative tumor margin delineation in clinical settings. These advantages are afforded while maintaining fast analysis times and subsequently high throughput. High resolving power ion mobility will enhance these advantages further, in particular for analyses requiring high confidence isobaric selectivity and detection.

Article highlights

  • Ion mobility improves selectivity in clinical analyses.

  • Integrated multidimensional separations with LC-IM-MS allow for exogenous isomeric compounds to be resolved.

  • Mass spectrometry imaging is improved by ion mobility selectivity.

  • Intraoperative deployment of mass spectrometry indicates tissue disease state.

  • Next generation high-resolution ion mobility allows for improved isomer separation for high confidence molecular annotation and quantitation.

Acknowledgments

The authors would like to acknowledge the Center for Innovative Technology at Vanderbilt University for providing supporting resources in preparation of this review.

Declaration of interests

The authors are unaware of any potential bias that may affect the objectivity of the review but do acknowledge collaborative arrangements with Agilent Technologies (Santa Clara, CA), Waters Corporation (Milford, MA), and MOBILion Systems (Chadds Ford, PA). The Vanderbilt University Center for Innovative Technology is designated as an Agilent Thought Leader Laboratory and a Waters Center of Innovation. J.A.M is a member of the Scientific Advisory Board for MOBILion Systems, which is a supplier of commercial ion mobility instruments. J.A.M. certifies that contributions are scientifically objective and are not influenced by his SAB participation.

Reviewer disclosures

Peer reviewers on this manuscript have no relevant financial or other relationships to disclose.

Additional information

Funding

This manuscript was funded by the National Institutes of Health, National Institute of Child Health and Human Development (NIH HHS) under award [R01HD102752].

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