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ABSTRACT
The present study was undertaken to characterize the Cr(VI)-resistant bacteria isolated from the chromite mine area of Sukinda, Odisha through 16S rRNA sequencing technique followed by homology search, secondary structure analysis. The 16S rRNA sequencing resulted in varied bacterial sequence lengths. Characterization of these isolated sequences showed the dominance of the members of the genera Bacillus, Lysinibacillus, and Staphylococcus followed by the members of other distinct genera such as Enterobacter, Curtobacterium, Kocuria, and Stenotrophomonas. Cr(VI)-tolerant study of these bacterial isolates exhibited comparatively higher resistance toward Cr(VI) at 500 mg L−1 concentration. Secondary-structural diversity among isolated sequences obtained from mfold prediction server varied with sequence-based diversity with some conserved pattern observed among the few Bacillus groups and Staphylococcus groups, but in other genera, the structural similarity is not observed. Multiple sequence alignment of all 16S rRNA sequences showed monomorphic and polymorphic sites in most of the sequences, whereas parsimony-informative sites were identified in a few bacterial isolates. Further, phylogenetic analysis of the isolated chromium-resistant 16S rRNA bacterial sequences along with the sequences retrieved from NCBI’s ENTREZ database was performed to study their divergence. Phylogenetic analysis revealed that chromate-resistant sequences were clustered into two distinct clades in maximum parsimony-based phylogenetic tree construction.