Mass Spectrometry and Integrated Virus Detection System Characterization of MS2 Bacteriophage

ABSTRACT

In this study, we demonstrate the effect of sample matrix composition of MS2 virus on its characterization by ESI-MS and IVDS. MS2 samples grown and purified using various techniques showed different responses on ESI-MS than that on IVDS. The LC-MS of the specific biomarker of MS2 bacteriophage from an infected Escherichia coli sample was characterized by the presence of E. coli proteins. The significant impact of sample matrix was observed upon identification of MS2 using a database search. Infected E. coli with MS2 showed a matching score indifferent from uninfected ones. Only purified MS2, using CsCl and analyzed by LS-MS, showed a positive match using the database search. However, the variation in MS2 sample matrix had no effect on the deification of MS2.

KEYWORDS :

• Bacteriophage
• Database
• Detection
• Electrospray Ionization
• Integrated Virus Detection System
• IVDS
• Mass Spectrometry
• MS2
• Online Sample Processing
• Proteomics
• Purification
• Separation
• Virus
• Virus Detection