https://orcid.org/0000-0001-7223-2626
Figures & data
Figure 1. Analysis the features and functions of lncRNA source genes
The top 20 results of KEGG pathway analysis corresponds to the lncRNA source genes.
Figure 2. qRT-PCR analysis of lncRNA expression
(a) Changes in lncRNA expression validated by qRT-PCR. Comparison results of the 5 lncRNAs using the qRT-PCR and RNA-seq. The vertical axis showed the mean fold change (log2 fold change) of each lncRNA. (b) Relative expression of lncRNAs. The expression profile of the lncRNA lnc_13814 in eight tissues of duck expressed as the mean ± SD.
Figure 3. Biomathematically predicted the target lncRNA-miRNA-mRNA network of lnc_13814
(a) The lncRNA-miRNA co-expression network of lnc_13814. (b) Parts molecular interactions of lnc_13814 with its miRNA and target genes.
Figure 4. Lnc_13814 serves as a sponge for miRNAs apla-mir-145-4 and apla-mir-25-42
(a) The luciferase activity detection results of lnc_13814 serves as a sponge for miRNA apla-mir-145-4; (b) The luciferase activity detection results of lnc_13814 serves as a sponge for miRNA apla-mir-25-42; (c) The lnc_13814 expression level was measured by qRT-PCR when apla-mir-145-4 mimic was transfected into GC cells; (d) The apla-mir-145-4 expression level was measured by qRT-PCR and apla-mir-1-13 inhibitor were transfected into GC cells, respectively.
Figure 5. Effect of lnc_13814 on proliferation and apoptosis on duck GCs
(a) Cell cycle arrest profiles upon lnc_13814 overexpression; (b) The expression of genes related to proliferation and apoptosis upon lnc_13814 overexpression; (c) The apoptosis results of lnc_13814 overexpression in GCs by EdU assay. (d) Apoptosis in each group detected by annexin V-FITC/PI double staining.
Figure 6. DDIT3 was a directed target gene of apla-miR-145-4
(a) The Putative apla-miR-145-4 binding site in the 3ʹUTR of DDIT3 mRNA (apla-miR-145-4 was also named as gga-miR-146 c-3p of chicken). (b) Sequence alignment of a putative apla-miR-145-4 binding site within the 3ʹUTR of DDIT3 mRNA. (c) Interaction between apla-miR-145-4 and DDIT3 was verified by luciferase report assay. (d) The effect of apla-miR-145-4 on DDIT3 expression in GCs which was detected by QPCR.
Figure 7. Lnc_13814 plays biological function in GCs by sponging apla-miR-145-4
(a) DDIT3 mRNA expression result when lnc_13814 overexpression construct and apla-miR-145-4 mimic was co-transfected into GC cells; DDIT3 mRNA expression level was measured by qRT-PCR when lnc_13814 overexpression construct was transfected into GC cells. (b) BCL2 and FAS mRNA expression levels were measured by qRT-PCR when lnc_13814 overexpression construct, lnc_13814 overexpression construct and apla-miR-145-4 mimic were co-transfected into GC cells.
Supplemental material
Supplemental Material
Download Zip (45 MB)Availability of data and materials
The data that support the findings of this study are openly available in NCBI Sequence Read Archive at https://www.ncbi.nlm.nih.gov/sra/PRJNA526427, SRA reference number PRJNA526427.
