Figures & data
Figure 1. A schematic hypothetical diagram of degradative autophagy, selective regulation of MIR224 and CCND1 and evidence of MIR224 and CCND1 coexistence in the autophagosome. (a) CCND1 is selectively recruited to the phagophore through the specific receptor SQSTM1. MIR224 is preferentially recruited to the phagophore by unknown factors. Both of the oncogenic factors are present in autophagosomes after selective recruitment; subsequent autophagosome fusion with the lysosome is followed by degradation. (b) Human liver cancer Hep 3B cells were treated with the autophagy inducer amiodarone. Coexistence of immunogold-labeled CCND1 (12-nm bead) and MIR224 (20-nm bead) after miRNA in situ hybridization in the purified autophagosomes was investigated using TEM. Scale bar: 100 nm.
