https://orcid.org/0000-0003-0685-5387
Figures & data
Figure 1. Eip93F is required for autophagosome and autolyosome formation in MB neuroblasts during late stages of pupal development. (a–f) Top, colored overlay of MB neuroblasts (white brackets) at the indicated times. (a,c,e) Wild-type control genotype: worGAL4,UAS-GFP-mCh-Atg8. (b,d,f) Eip93F knockdown (E93 RNAi) genotype: worGAL4,UAS-GFP-mCh-Atg8,UAS-E93 RNAi. Below, colored overlay of cropped, maximum intensity projection of MB neuroblast shown above, with single channel grayscale images below. White arrows indicate autophagomes (mCh and GFP double positive) and arrowheads indicate autolysosomes (mCh only). (a–f) Scale bar: 10 μm. scrib (scribble, blue) is a membrane marker to denote the MB neuroblast. APF, after pupal formation. Reprinted from Current Biology, 29(5), Pahl, M.C., Doyle, S.E., and Siegrist, S.E., E93 Integrates neuroblast intrinsic state with developmental time to terminate MB neurogenesis via autophagy, Figure 4A-F, 2019, with permission from Elsevier.
