Figures & data
Figure 1. Chitin-triggered ROS production in barley. (A) Typical ROS production measurement in response to chitin in barley. Normalized relative light units (RLU) are shown over the measured time course. Chitin elicitors were added to leaf discs at 0 min and ROS-dependent luminol luminescence recorded over time. Data show relative light units (RLU) corrected after subtraction of leaf disc-specific background (recorded for 5 minutes before elicitation) and average mock treatment associated blanks (n = 8). Error bars show standard error over the mean (n = 8). For methodology, see Scheler et al. (2016).Citation11
Figure 2. Comparison of Arabidopsis and barley MAP kinases. (A) Arabidopsis MPKs activated in response to chitin. MAPK activation of wild type and lyk1 (synonym cerk1) mutants was analyzed by immunoblotting using an antibody against phosphorylated TEY-motif containing MPKs (@pTEpY). For methodology see Ranf et al. (2015)Citation25 and Scheler et al. (2016).Citation11 (B) Barley MPKs activated in response to chitin were detected by @pTEpY immunoblotting. AB, amidoblack total protein staining as loading control. (C) Percent identity matrix of Arabidopsis TEY-MPKs activated in PTI and related barley proteins. Arabidopsis proteins (AtMPKs) were compared by reciprocal BLAST searches to high confidence predicted barley proteins and most similar barley (Hv) proteins containing a TEY-motif were selected as putative orthologs.
