MiR-140 targets lncRNA FAM230B to suppress cell proliferation in acute myeloid leukemia running title: MiR-140 targets FAM230B in AML

Figures & data

Table 1. Clinical data of 60 AML patients and 60 controls included in this study.

Features	AML (n = 60)	Control (n = 60)	P value
Age			>0.05
<60	38	38
≥60	22	22
Gender			>0.05
Male	27	27
Female	33	33
WBC (×10^9/l)			<0.0001
<100	34	60
≥100	26	0
HB (g/l)			<0.0001
<60	37	60
≥60	23	0
PLT (×10^9/l)			<0.05
<30	40	49
≥30	20	11
Bone Marrow Blasts (%)			<0.0001
<60	35	0
≥60	25	60
FAB Subtypes			na
M0	1	na
M1	1	na
M2	28	na
M3	8	na
M4	14	na
M5	8	na
Modified MRC risk stratification			na
Favorable	23	na
Intermediate	20	na
Adverse	17	na
ELN risk stratification (non-M3)
Favorable	25	na	na
Intermediate I and II	15	na
Adverse	20	na

Notes: WBC, white blood cells; HB, hemoglobin; PLT, platelet count; na, not available.

Figure 1. Differential expression of FAM230B and miR-140 in AML.

Note: The differential expression of FAM230B (A) and miR-140 (B) in BMMNCs from both AML patients and controls were analyzed through RNA isolations, followed by RT-PCRs. Correlations between FAM230B and miR-140 across both AML (C) and Control (D) samples were analyzed using Pearson’s correlation coefficient. **p < 0.01.

Figure 2. Direct interaction between FAM230B and miR-140 and subcellular FAM230B localization in KG-1 and THP-1 cells.

Note: IntaRNA 2.0 was used to predict the potential interaction between FAM230B and miR-140 (A). RNA pull-down assay was carried out to study the direct interaction between FAM230B and miR-140 (B). Cellular fractionation assay was performed to determine the subcellular localization of FAM230B in KG-1 and THP-1 cells (C). **p < 0.01.

Figure 3. The role of miR-140 in FAM230B expression.

Note: FAM230B or miR-140 were overexpressed in KG-1 and THP-1 cells, and FAM230B and miR-140 overexpression was confirmed every 24 h until 96 h (A). The role of FAM230B expression in miR-140 expression (B) and the role of miR-140 expression in FAM230B expression (C) in AML cells were analyzed with RT-qPCRs. *p < 0.05.

Figure 4. The role of miR-140 and FAM230B in AML cell proliferation.

Note: BrdU assays were performed to study the role of miR-140 and FAM230B in the proliferation of both KG-1 and THP-1 cells. *p < 0.05.