Interactions between Lotus japonicus genotypes and arbuscular mycorrhizal fungi

Figures & data

Figure 1.  Percentage of root length colonized by four individual fungi in wild-type ‘Gifu’ and in three symbiotic mutants of Lotus japonicus. Colonization in mutants was only by extraradical and intraradical hyphae. Bar indicates LSD at p<0.05.

Table I Percentage root length colonized by external hyphae, appressoria, internal hyphae, arbuscules and vesicles of four different AM fungi.

Mutant	AMF species	External hyphae	Appressoria	Internal hyphae	Arbuscules	Vesicles
“Gifu” B-129	Glomus sp. R-10	13.8	0.9	16.5	39.2	8.3
	Glomus etunicatum	7.4	0.8	22.6	27.0	5.5
	Glomus intraradices	11.3	0.8	23.1	27.0	14.3
	Gigaspora margarita	7.2	0.8	33.1	14.3	0*
Ljsym72	Glomus sp. R-10	9.4	1.7	0	0	0
	Glomus etunicatum	18.1	1.9	0	0	0
	Glomus intraradices	20.9	1.9	0	0	0
	Gigaspora margarita	6.0	1.6	0	0	0*
Ljsym 71-1	Glomus sp. R-10	8.8	7.3	3.7	0.9	1.3
	Glomus etunicatum	8.7	3.8	8.7	2.8	0.3
	Glomus intraradices	18.0	5.6	12.0	11.2	0.3
	Gigaspora margarita	11.9	2.2	11.5	6.9	0*
Ljsym71-2	Glomus sp. R-10	9.1	5.0	8.9	4.7	0
	Glomus etunicatum	7.7	2.2	5.0	4.1	0
	Glomus intraradices	22.0	6.9	10.2	5.8	1.1
	Gigaspora margarita	9.0	5.1	7.0	0	0*
	LSD (P < 0.05)	7.5	2.5	5.2	7.6	1.3
ANOVA
	Mutant	NS	***	***	***	***
	AMF	***	*	**	**	***
	Mutant x AMF	*	*	**	***	***
*Gigaspora margarita do not produce vesicles naturally.
Significance of the effect (NS, not significant, *P < 0.005, **P < 0.01, ***P < 0.001).

Figure 2.  Light microscopy of cleared and trypan blue stained roots of wild-type ‘Gifu’ (Figures A–D) and mutant Ljsym 72 (Figures E–H) of Lotus japonicus, colonized by Glomus sp. R-10 (A, E), Glomus etunicatum (B, F), Glomus intraradices (C, G) and Gigaspora margarita (D, H). eh, external hypahe, ap, appressoria, ih, internal hyphae, ar, arbuscules, v, vesicles. Bar represents 50 µm.

Figure 3.  Light microscopy of cleared and trypan blue stained roots of mutant Ljsym 71-1 (Figures A–D) and mutant Ljsym 71-2 (Figures E–H) of Lotus japonicus, colonized by Glomus sp. R-10 (A, E), Glomus etunicatum (B, F), Glomus intraradices (C, G) and Gigaspora margarita (D, H). ap, appressoria, ih, internal hyphae. Bar represents 50 µm.