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ORIGINAL ARTICLES

In vitro allelopathic potential of Leonurus sibiricus L. leaves

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Pages 39-48 | Received 23 Aug 2007, Published online: 04 Apr 2008

Figures & data

Figure 1.  Effects of different doses of aqueous (a) and methanol (b) extracts of Leonurus sibiricus leaves on root elongation of Raphanus sativus, 120 h after sowing.

Figure 1.  Effects of different doses of aqueous (a) and methanol (b) extracts of Leonurus sibiricus leaves on root elongation of Raphanus sativus, 120 h after sowing.

Figure 2.  Effects of different doses of aqueous (a) and methanol (b) extracts of Leonurus sibiricus leaves on root elongation of Lactuca sativa, 120 h after sowing.

Figure 2.  Effects of different doses of aqueous (a) and methanol (b) extracts of Leonurus sibiricus leaves on root elongation of Lactuca sativa, 120 h after sowing.

Figure 3.  Effects of different doses of aqueous (a) and methanol (b) extracts of Leonurus sibiricus leaves, on root elongation of Lepidium sativum, 120 h after sowing.

Figure 3.  Effects of different doses of aqueous (a) and methanol (b) extracts of Leonurus sibiricus leaves, on root elongation of Lepidium sativum, 120 h after sowing.

Table 1 Effects of different doses of aqueous and methanol extracts on Leonurus sibiricus on the germination of seeds of Raphanus sativus, Lactuca sativa and Lepidium sativum, 120 h after sowing. Data are the mean of three experiments±SD.

Table 2 HPLC quantification of major flavonoids of the aqueous and methanol extracts of Leonurus sibiricus. Data are expressed in mg g−1 of dry leaves±SD.

Table 3 Biological activtities of Sephadex fractions (1 g l−1) from the methanol extract of Leonurus sibiricus against germination and radical elongation of Raphanus sativus, Lactuca sativa and Lepidium sativum.

Table 4 Seed germination of Lactuca sativa under different concentrations of isolated flavonoids.

Figure 4.  Chromatographic profile of aqueous (I) and methanol (II) extracts of leaves of Leonurus sibiricus showing the main flavonoidic compounds.

Figure 4.  Chromatographic profile of aqueous (I) and methanol (II) extracts of leaves of Leonurus sibiricus showing the main flavonoidic compounds.

Figure 5.  Effects of different concentrations of the flavonoids isolated from L. sibiricus leaves on root elongation of Lactuca sativa, 120 h after sowing. A = quercetin-3-O-α-L-rhamnopyranosyl-(1 > 6)-β-D-galactopyranoside; B = rutin; C = hyperin; D = isoquercetrin; E = 3′-OH-genkwanin; F = genkwanin; G = quercetin.

Figure 5.  Effects of different concentrations of the flavonoids isolated from L. sibiricus leaves on root elongation of Lactuca sativa, 120 h after sowing. A = quercetin-3-O-α-L-rhamnopyranosyl-(1 > 6)-β-D-galactopyranoside; B = rutin; C = hyperin; D = isoquercetrin; E = 3′-OH-genkwanin; F = genkwanin; G = quercetin.

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